摘要
采用高效液相色谱-蒸发光散射检测器(HPLC-ELSD)测定不同厂家中/长链脂肪乳注射液中溶血磷脂酰胆碱(LPC)的含量。采用Lichrospher Diol色谱柱(250mm×4.6mm,5μm),以正己烷-异丙醇-水(29.5:59:11.5,V/V/V)为流动相A,正己烷-异丙醇(43:57,V/V)为流动相B,进行梯度洗脱,流速1mL/min,漂移管温度85℃,气体流速2.7L/min。结果溶血磷脂酰胆碱在0.299—2.99mg/mL浓度范围内线性关系良好(r=0.9999),平均回收率为99.62%,RSD为0.89%。不同厂家产品中溶血磷脂酰胆碱的含量为0.92—1.34mg/mL。方法准确、方便,可以准确测定中/长链脂肪乳注射液中溶血磷脂酰胆碱的含量。
The contents of lyso-phosphatidylcholine （LPC） in MCT/LCT fat from different manufacturers were determined by HPLC-ELSD. The emulsion injection lichrospher Diol （250minX4.6ram, 5μm） column was used. The mobile phase were A. n-hexane-isopropanol-water （29.5 ： 59 ： 11.5,V/V/V） and B：n-hexane-isopropanol （43 ： 57,V/V） with gradient elution. The flow rate was lmL/min. The temperature of drift tube was 85℃ and the gas flow rate was 2. 7L/min. There was a good linear relationship for LPC in the range of 0. 299--2. 99mg/mL （r=0. 9999）. The average recovery was 99. 620//oo and RSD was 0. 89%. The concentrations of LPC in MCT/LCT fat emulsion injection from different manufacturers were from 0. 92mg/mL to 1.34mg/mL. The method is accurate and convenient,and could be suitable for the determination of the content of LPC in MCT/LCT fat emulsion injection.
出处
《光谱实验室》
CAS
CSCD
2012年第4期2585-2588,共4页
Chinese Journal of Spectroscopy Laboratory
