摘要
目的调查本院耐亚胺培南鲍曼不动杆菌(IRAB)的分子流行特点,初步研究其对碳青霉烯类抗菌药物耐药的分子机制。方法收集2009年11月至2010年1月不同临床科室分离的IRAB非重复株共54株,用Vitek 2 Compact全自动微生物分析仪进行药敏试验;用脉冲场凝胶电泳(PFGE)分析IRAB的同源性;PCR及DNA测序检测碳青霉烯酶和16S rRNA甲基化酶相关耐药基因型。结果 54株细菌PFGE可分为A、B两个型,其中A型42株,占77.8%,在各科室均有分布,为主要的流行型别;B型10株,占18.5%,仅在神经内、外科的ICU分布。全部54株菌均携带OXA-66基因,53株菌携带OXA-23基因及其上游插入序列ISAba1,41株菌携带16S rRNA甲基化酶armA基因。结论医院临床各科室存在A型IRAB的克隆播散,而B型IRAB仅在神经内、外科ICU流行。OXA-23基因及其上游插入序列ISAba1是最主要的耐碳青霉烯类抗菌药物机制,armA型16S rRNA甲基化酶基因在IRAB中广泛分布。
Objective To investigate the molecular prevalent feature of imipenem-resistant Acinetobacter baumannii （IRAB） in our hospital, and analyze its molecular mechanism of resistance to earbapenems. Methods Fifty-four non-repetitive IRAB strains isolated from different clinical departments were collected from November 2009 to January 2010. The antimicrobial susceptibility test was per- formed with Vitek-2 Compact system. The homology of IRAB was analyzed by pulsed field gel electrophoresis （PFGE）. The related re- sistance genetypes of carbapenemase and 16S rRNA methylase were detected by PCR and DNA sequencing. Results Fifty-four IRAB strains were distributed into type A and B according to PFGE results. Among the 54 strains, 42 strains （77.8%） belonged to type A which was the main prevalent type and distributed in all the departments, and 10 strains （ 18.5% ） belonged to type B which only dis- tributed in the intensive care units （ICU） of neurology and neurosurgery departments. OXA-66 gene was carried by all the 54 strains, OXA-23 gene and its upstream insertion sequence ISAbal was carried by 53 stains, and the armA gene of 16S rRNA methylase was car- ried by 41 strains. Conclusion Type A of IRAB was disseminated widely in the clinical departments of our hospital, while type B spread in ICU of neurology and neurosurgery departments. The existence of OXA-23 gene and its upstream insertion sequence ISAbal was the main cause leading IRAB to resist carbapenems. 16S rRNA methylase armA gene was widely distributed in IRAB strains.
出处
《临床检验杂志》
CAS
CSCD
北大核心
2012年第6期463-465,共3页
Chinese Journal of Clinical Laboratory Science
