摘要
目的观察角膜溃疡多层羊膜移植术后羊膜与角膜早期组织病理学形态与临床特点。方法角膜溃疡患者23例(23眼),以多层羊膜填塞溃疡面,单层羊膜覆盖角膜表面,10-0线间断缝合于角膜缘。角膜组织行病理学检查,裂隙灯观察角膜溃疡变化,并观察术后并发症。结果 21例患者角膜溃疡愈合;2例患者溃疡穿孔再次行光学性角膜移植术,其中1眼继发青光眼。无其他并发症发生。HE染色羊膜呈染色均一结构,18d多层羊膜排列整齐,层次清晰,角膜基质内少量炎性粒细胞浸润,羊膜表面角膜上皮化;30d多层羊膜彼此融合,层次不清,羊膜与角膜基质界限模糊,少量新生血管形成,角膜厚度均一。结论多层羊膜移植后羊膜与角膜基质可有效整合,增加角膜厚度,阻止溃疡穿孔,抑制炎症,促进溃疡愈合。
Objective To investigate the early histopathology of amniotic membrane and corneal integration and clinical characteristic of multilayer amniotic membrane transplantation in corneal ulcers.Methods Twenty-three eyes of 23 consecutive patients were enrolled.Multiple layers of amniotic membrane were applied over the ulcer bed to fill the ulcer crater and held in place with an overlying amniotic membrane patch,which was anchored to the surrounding cornea with 10-0 nylon interrupted sutures.The histopathology of corneal tissue was observed.The complication and progression of corneal ulcers was observed by slit lamp.Results The corneal ulcers of 21 eyes were healed,and the penetrating corneal transplantation was made in 2 eyes,in which the secondary glaucoma was occurred in 1 eye,no other complication was observed.Uniform structure of amniotic membrane,epithelialization over the amniotic membrane and small quantity inflammatory cells were observed at 18 days after transplantation.But indistinct structure of amniotic membrane,little new cornea vessels,integration between cornea stroma and amniotic membrane were presented at 30 days after transplantation.Conclusion The multilayer amniotic membrane in transplantation can integrate into the corneal stroma,increase the corneal thickness,prevent the perforation of corneal ulcers,inhibit the inflammation and promote the ulcer healing.
出处
《眼科新进展》
CAS
北大核心
2012年第8期750-752,共3页
Recent Advances in Ophthalmology
关键词
角膜溃疡
多层羊膜
组织病理
临床分析
corneal ulcers
multilayer amniotic membrane
histopathology
clinical analysis
