摘要
目的探讨临床分离的多重耐药铜绿假单胞菌β-内酰胺酶相关耐药基因及氨基糖苷类修饰酶基因。方法采用MicroScan40微生物全自动鉴定系统微量肉汤稀释法测定菌株的耐药性。采用聚合酶链反应(PCR)方法检测β-内酰胺酶基因型及氨基糖苷类修饰酶基因型。结果 32株铜绿假单胞菌除对哌拉西林/他唑巴坦和替卡西林/克拉维酸耐药率分别为75.9%和93.1%外,对其余抗菌药物耐药率均为100%。氨基糖苷类修饰酶基因检出30株(93.7%),aac(3)-Ⅰ、aac(3)-Ⅱ、aac(6)-Ⅰ、aac(6)-Ⅱ、ant(3)-Ⅰ和ant(2)-Ⅰ基因阳性率分别为9.3%、90.6%、12.5%、90.6%、90.6%和0,检出β-内酰胺酶基因28株(87.5%),TEM、DHA、OXA和IMP基因阳性率分别为62.5%、43.7、21.8%和3.1%,未检出VIM、SHV和CTX-M-9,检出OprD2基因缺失23株(71.8%)。结论广州地区多重耐药铜绿假单胞菌携带多种氨基糖苷类修饰酶基因和β-内酰胺酶基因。
Objective To investigate the resistant genes of β-1actamases （BLs） and aminoglycoside-modifying enzyme （AMEs） by multidrug-resistant Pseudomonas aeruginosa （PAE） isolatfd from clinical specimens inhospitat. Methods The antimicrobial resistance was tested by Dade MicroScan40. The β-1actamase gene and aminoglycoside-modifying enzyme gene car- ried on multidrug-resistant Pseudomonas aeruginosa were detected by PCR. Results The resistant rates of 32 PAE to TZP andTCC were 75.9% and 93. 1%, The resistant rates of other antibiotics were 100%. The prevalence of AMEs was 30 strains （93.7%）, The positive rates ofaac （3） -Ⅰ, aac （3） -Ⅱ, aae （6） -Ⅰ, aac （6） -Ⅱ, ant （3） -Iand ant （2） -Ⅰ were 9. 3%, 90. 6%, 12. 5%, 90. 6% , 90. 6% and 0%. The prevalence of BLs was 28 strains （ 87. 5% ）, The positive rates of TEM, DHA, OXA and IMP were 62. 5%, 43.7%, 21.8%and3.1%, VIM, SHV and CTX-M-9 genes were not de- tected. Loss of OprD2 porin was identified in 23 （71.8%） . Conclusion The multidrug-resistant Pseudomonas aeruginosa carrying a variety of aminoglycoside-modifying enzyme and β-1actamase genes.
出处
《广州医药》
2012年第4期51-53,共3页
Guangzhou Medical Journal
关键词
铜绿假单胞菌
多重耐药
耐药基因
Pseudomonas aeruginosa
Multi-drug resistance
Resistant gene
