摘要
目的:建立酶解液相色谱法测定硫酸软骨素钠的含量。方法:使用硫酸软骨素ABC酶酶解硫酸软骨素钠,使用强阴离子交换高效液相色谱测定酶解产生的二糖,通过计算二糖峰面积总和确定硫酸软骨素钠的含量。色谱柱为Zorbax SAX(250 mm×4.6 mm,5μm);流动相为pH 3.5的水和pH 3.5的2 mol.L-1的氯化钠溶液,流速为1.0 mL.min-1,检测波长为232 nm,柱温为40℃。结果:硫酸软骨素钠的线性范围为0.25～1.50 mg.mL-1,r=0.9999;精密度RSD为0.3%;回收率为101.2%,RSD为1.6%(n=5);最低检测限为4.0μg.mL-1,定量限为11.9μg.mL-1。结论:该方法具有专属性好,灵敏度高,重现性好的优点。
Objective ：To estabhsh enzymolysis -HPLC method for determining of the content of chondroitin sul- fate sodium. Methods： Chondroitin sulfate sodium was first digested by chondroitinase ABC. The CS disaccha- rides produced were analyzed by SAX - HPLC and CS content was quantified by calculating the total peak areas of the disaccharides. Zorbax SAX column（250 mm ×4.6 mm,5 μm）was adopted the mobile phase of water （pH 3.5 ） and 2 mol . L^-1NaCl（ pH 3.5 ） at 40 ~C with flow rate of 1.0 mL .min^-1, detecting wavelength being 232 nm. Results： The calibration curve was linear in the range of 0. 25 - 1.50 mg . mL^-1 with correlation coefficient of 0. 9999. The method precision RSD was 0. 3%. The average recovery was 101.2% and its RSD was 1.6% （n =5）. The low detection limit was 4.0 μg . mL^-1 and the quantitative limit was 11.9 μg .mL^-1. Conclusion： This method is reproducible, sensitive and suitable for the determination of the content of chon- droitin sulfate sodium.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2012年第7期1246-1248,1258,共4页
Chinese Journal of Pharmaceutical Analysis
关键词
硫酸软骨素钠
酸性黏多糖
酶解液相色谱法
含量测定
标准提高
chondroitin sulfate sodium
acidic mucopolysaccharide
enzymolysis - HPLC
assay
standard improve-merit
