摘要
以番茄叶片为材料提取小RNA,比较分析UNlQ-10柱式Trizol总RNA抽提试剂盒+LiCl法,常规Tr-izol+LiCl法和改进的Trizol法+LiCl法的提取效果。结果表明,改进的Trizol+LiCl法能从番茄叶片中提取高质量的小RNA,总RNA琼脂糖凝胶电泳条带完整清晰,核酸蛋白测定仪显示RNA的浓度为1 026.8ng/μL,A260/A280比值为2.00;A260/A230比值为2.31。15%聚丙烯酰胺-7mol/L尿素凝胶电泳显示小RNA带型区域清晰。用该方法提取的高质量的番茄叶片小RNA符合RT-PCR,Northern blot等分子生物学试验的标准。
This experiment used tomato leaf to extract small RNA. UNIQ-10 Column Trizol Extraction Kit＋LiCl method, a general Trizol＋ LiCl method and the improved Trizol ＋LiCl method were compared on the extraction effect. The results showed that the improved Trizol＋LiCl method could extract high-quality small RNA from tomato leaves, Agarose gel electro- phoresis of total RNA showed clear and complete strips, nucleic acid protein detector measured the 1 026.8 ng/μL in concentra tion, 2.00 for A260/A280 and 2.31 for A260/A230. 15% polyacrylamide--7 M urea gel electrophoresis showed clear small RNA strips area. The high-quality small RNA from tomato leaves by improved Trizol＋ LiCl method met the criterion for small RNA Northern blot and other molecular biology experiments.
出处
《北京农学院学报》
2012年第3期4-6,共3页
Journal of Beijing University of Agriculture
基金
国家自然科学基金项目(No.31171952)
北京市自然科学基金项目(No.6092005)
关键词
番茄
叶片
小RNA提取方法
Solanum lycopersicum
leaf
small RNA extraction methods
