摘要
目的:探索大鼠局灶性脑缺血再灌注损伤最严重的时间点,确定开展发病机制及药理学研究最合适的时间窗。方法:制造线栓法阻塞大脑中动脉致大鼠局灶性脑缺血再灌注损伤模型(MCAO),缺血后2h再灌注,术后动态观察大鼠的状态,神经行为学,脑梗死比率和血液中的相关生化指标。结果:模型大鼠的病理损害具有明显的时相性,随着时间的推移逐渐加重,于48h达到高峰,96h时病理损害仍然存在,但明显减弱;在脑缺血第48h时,脑梗死比率、神经行为学和血清中脂质过氧化指标等病理损害均比较严重,且动物的体重和状态开始下降。结论:MCAO大鼠脑缺血后第48h时最适宜于开展发病机制及药理学研究,且雄性SD大鼠更适宜制作该模型。
To investigate time points of the most serious state for a rat model of focal cerebral ischemia-reperfusion injuries, and to i- dentify the most appropriate time window for the pathogenesis and pharmacology study. Methods ： The focal cerebral ischemia-reperfusion in- jury model was made by raiddle cerebral artery occlusion （MCAO） in rats. After reperfusion following 2h of cerebral isehemia the states, neural behaviors, cerebral infarction ratio and tile biochemical indexes in blood of rats were dynamically detreted. Results： The pathological injuries of rat models changed according to cerebral ischemia time. With the passage of time gradually deteriorated, the most serious state was at 48h, and the pathological injuries was still existed but weakened obviously at 9611. At 48h of cerebral ischemia for 48h, cerebral infarction ratio, neural behaviors, and the lipid peroxide indexes in serum of pathological damage were relatively serious, and the animal weight andstate began to decrease. Conclusion： The best time to conduct the pathogenesis and pharmacology study was 48h of cerebral ischemia, and male SD rats were more suitable for this model.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2012年第3期116-120,共5页
Pharmacology and Clinics of Chinese Materia Medica
基金
国家自然科学基金项目(基于结构方程模型的民族药灯盏细辛防治缺血性中风的PK-PD相关性研究
№81102895)
教育部2011年度高等学校博士学科点专项科研基金(基于PK-PD结构方程模型的民族药灯盏细辛防治缺血性中风的作用机制研究
№20115132120007)
