期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

人脐带间充质干细胞移植对IgA肾病小鼠肾功能的影响 被引量：2

下载PDF
导出
摘要 目的观察人脐带间充质干细胞移植对IgA肾病小鼠肾功能及肾组织的影响,并探讨其可能机制。方法 SPF级雄性昆明小鼠60只,随机分为正常对照组、模型组和治疗组,各20只。模型组和治疗组用牛血清白蛋白灌胃、CCl4皮下注射、脂多糖尾静脉注射的方法制作IgA肾病模型。造模成功后,治疗组经尾静脉注射人脐带间充质干细胞0.5 mL(含5×105个间充质干细胞),对照组、模型组经尾静脉注射生理盐水0.5 mL。于治疗前及治疗后第72、1、56天,检测小鼠肾功能指标(血Cr、BUN、总蛋白及24 h尿蛋白);处死小鼠取肾组织,行Masson染色观察肾组织病理变化,用免疫组化法检测肾组织中的VEGF。结果模型组小鼠均出现蛋白尿和血尿(15只小鼠出现镜下血尿,5只出现肉眼血尿);治疗组于第21天小鼠血尿有所消失。与模型组治疗后比较,治疗组肾组织Masson染色显示纤维化程度明显减轻、VEGF表达增强。治疗后第72、1、56天,治疗组SCr2、4 h尿蛋白较模型组明显改善(P<0.05或0.01),在治疗后第56天治疗组BUN较模型组明显下降(P<0.01)。结论人脐带间充质干细胞移植对IgA肾病小鼠肾功能具有保护作用,其机制可能与上调肾组织中VEGF表达有关。
作者 孙洞箫 王志奇 周盾
机构地区 辽宁医学院附属第一医院
出处 《山东医药》 CAS 2012年第26期36-38,共3页 Shandong Medical Journal
基金 辽宁省科技厅科学技术计划项目(2010225034)
关键词 肾小球疾病 IGA肾病 脐带间充质干细胞 干细胞移植 肾功能 血管内皮生长因子 小鼠
分类号 R692 [医药卫生—泌尿科学]
  • 相关文献

参考文献16

  • 1Tang YL. Autologous mesenchymal stem cells for post-ischemic myocardial repair[ J]. Methods Mol Med, 2005, 112 ( 15 ) : 183- 192.
  • 2Yokoo T, Ohashi T, Utsunomiya Y, et al. Genetically modified bone marrow continuously supplies anti-inflammatory cells and suppresses renal injury in mouse good pasture syndrome [ J ]. Blood, 2001,98( 1 ) :57-64.
  • 3汤颖,娄探奇,成彩联,彭晖,关伟明.实验性IgA肾病模型的改进[J].中山大学学报（医学科学版）,2006,27(2):184-187. 被引量：106
  • 4Hill GS, Karoui KE, Karras A, et al. Focal segmental glomerulo- sclerosis plays a major role in the progression of IgA nephropathy [J]. Kidney Int,2011,79(6) :635-642.
  • 5岳丽玲,于海涛,郑立红.胚胎干细胞的研究现状及临床应用前景[J].中国优生与遗传杂志,2005,13(1):4-5. 被引量：10
  • 6Bartholomew A, Sturgeon C, Siatskas M, et al. Mesenchymal stem cells suppress lymphocyte proliferation in vitro and prolong skin graft survival in vivo[J]. Exp Hematol, 2002,30( 1 ) :42-48.
  • 7Devine SM, Bartholomew AM, Mahmud N, et al. Mesenchymal stem cells are capable of homing to the bone marrow of non-human primates following systemic infusion[ J]. Exp Hematol, 2001,29 (2) :244-255.
  • 8Ortiz LA, Gambelli F, McBride C,et al. Mesenchymal stem cell engraftment in lung is enhanced in response to bleomyein exposure and ameliorates its fibrotic effects [ J ]. Proc Natal Acidic USA, 2003,100 ( 14 ) : 8407-8411.
  • 9Rojas M, Xu J, Woods CR, et al. Bone marrow-derived mesenchy- real stem ceils in repair of the injured lung [ J ]. Am J Respire Cell Mol Biol, 2005,33 ( 2 ) : 145-152.
  • 10Ortiz LA, Gambelli F, McBride C, et al. Mesenchymal stem cell engraftment in lung is enhanced in response to b|eomycin exposure and ameliorates its fibrotic effects [ J ]. Proc Natl Acad Sci USA, 2007,1 O0 ( 14 ) : 8407-8411.

二级参考文献18

  • 1李江涛,许晨,李素,孙向华,马骏.雷公藤单体T4治疗IgA肾病的实验研究[J].上海医学,2003,26(S1):19-22. 被引量：9
  • 2刘震,周树录,谭建三,王峥,王增贵,张晓东,余运成.大鼠系膜增殖型肾小球肾炎模型的改进[J].华西医科大学学报,1996,27(2):182-184. 被引量：58
  • 3Kaji EH,Leiden JM.Gene and stem cell therapies[J]. JAMA,2001,285:545-550.
  • 4Draper JS,Pigott C,Thomson JA,et al.Surface antigens of human embryonic stem cells:changes upon different tiation in culture[J].J Anat,2002,200:249-258.
  • 5Thomson JA,Itskovitz EJ,Shapiro SS,et al.Embryonic stem cell line derived from human blastocysts[J].Science,1998,282:1145-1147.
  • 6Shamblott MJ,Axelman J,Gearhart JD.Derivation of potential stem cells from cultured human primordial germ cells[J].Proc Natl Acad Sci USA,1998,95:13726-13731.
  • 7Evans MJ,Kaufman MH. Establishment in culture of pluripotential cells from mouse esbryos[J].Nature,1981,292:154-159.
  • 8Benjamin E Reubinoff,Martin F Pera,Chui-Yee FONG,et al.Embryonic stem cell lines from human blastocysts:somatic differentiation in vitro[J].NAT Biotechnol,2000,18(4:399-404).
  • 9Solter D,Gearhard J.Enhanced:Putting stem cells to work[J].Science,1999,283(5407)1468-1470.
  • 10Marshall E.Claim of human-com embeyo greeted with skepticism[J].Science,1998,282(5406):1390-1399.

共引文献114

同被引文献32

  • 1刘弘光,夏鲲,刘晓玉,庞希宁.移植骨髓间充质干细胞治疗大鼠糖尿病的研究[J].中国组织化学与细胞化学杂志,2007,16(1):104-109. 被引量：8
  • 2Friedenstein A J, Chailakhyan R K, Latsinik N V, ei al.Stromal cells responsible for transferring the microenviron-ment of the hemopoietic tissues. Cloning in vitro and re-transplantation in vivo [ J ]. Transplantation, 1974 , 17(4):331-340.
  • 3Chai Y C, Roberts S J,Desmet E , et al. Mechanisms ofectopic bone formation by human osteoprogenitor cells onCaP biomaterial carriers [ J ]. Biomaterials, 2012 , 33(11):3127-3142.
  • 4Polchow B,Kebbel K, Schmiedeknecht G,et al. Cryopr-eservation of human vscular umbilical cord cells undergood manufacturing practice conditions for future cellbanks[ J]. J Transl Med, 2012,10 :98.
  • 5Mitchell K E,Weiss M L, Mitchell B M , et al. Matrixcells from Wharton's jelly form neurons and glia[ J]. StemCells, 2003,21(1) :50-60.
  • 6Romanov Y A, Svintsitskaya V A, Smirnov V N. Search-ing for alternative sources of postnatal human mesenchy-mal stem cells ; candidate MSC-like cells from umbilicalcord [J]. Stem Cells, 2003,21(1) :105-110. ”.
  • 7Seda Tigli R,Ghosh S, Laha M M, e. al. Cortiparativechondrogenesis of human cell sources in 3D scaffolds[ J].J Tissue Eng Regen Med, 2009 ,3(5) :348-360.
  • 8Baffert F, Le T, Thurston G, et al. Angiopoietin-1 de-creases plasma leakage by reducing number and size ofendothelial gaps in venules [ J ]. Am J Physiol Heart CircPhysiol, 2006,290(1) :107-118.
  • 9Cao Y, Sun Z,Liao L, et al. Human adipose tissue-de-rived stem cellsdifferentiate into endothelial cells in vitroand improve postnatal neovascularization in vivo[ J ] . Bio-chemical and Biophysical Research Communications,2005,332(2) :370-379.
  • 10Kim S M,Lim J Y , Park S I, ei al. Gene therapy usingTRAIL-secreting human umbilical cord blood-derivedmesenchymal stem cells against intracranial glioma [ J ].Cancer Res, 2008 ,68(23 ) :9614-9623.

引证文献2

二级引证文献7

山东医药
2012年 第26期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部