摘要
以龙凤竹[Pedilanthus tithymaloides(L.)Poit var.nanus Dressler]茎段为外植体,进行愈伤组织诱导和再分化试验。在已筛选出的培养基中添加不同浓度的Ca2+进行试验,以确定诱导和再分化培养基中的最适的Ca2+浓度。结果表明,适度增加Ca2+浓度可促进龙凤竹愈伤组织的诱导和再分化,但当Ca2+浓度超过15 mmol/L时,促进效果显著降低。
Taking the stems of Pedilanthus tithymaloides（L.） Poit var.nanus Dressler as explants,experiments on callus induction and redifferentiation were conducted.The effect of Ca2＋ concentration added to the induction and redifferentiation medium on tissue culture efficiency was studied.The results showed that frequency of induction and redifferentiation could be increased with suitable Ca2＋ concentration.However,when the concentration of Ca2＋ was higher than 15 mmol/L,the promotion effect was significantly reduced.
出处
《湖北农业科学》
北大核心
2012年第12期2604-2605,共2页
Hubei Agricultural Sciences
基金
国家自然科学基金项目(31000410)
陕西省教育厅2009年度科学研究计划项目(09JK434)
