摘要
以玉米粉和麦麸为基质,采用固态发酵技术发酵饲用植物乳杆菌(Lactobacillus plantarum)。以活菌数为指标,设计单因素和L9(34)正交试验优化培养条件和培养基配方。结果表明,植物乳杆菌固态培养的最适条件为接种量10%,37℃静置发酵48 h。最适宜的固体发酵培养基中各营养成分的质量分数分别为麸皮59.5%、玉米粉35.0%、葡萄糖2.0%、乳糖1.0%、蛋白胨1.5%、酵母膏1.0%。培养结束后乳杆菌活菌数可达100.5×108CFU/g(鲜基)。
Using corn flour and wheat bran as ground substances, solid-state fermentation technique was conducted to ferment Lactobacillus plantarum. Taking the number of living bacteria as indicator, single factor tests and L9（34） orthogonal experiment were adopted to optimize the fermentation conditions and formula of medium. The results showed that the optimal culture con- ditions for L. plantarum were inoculation amount of 10% and fermenting for 48 h at 37 ℃. The optimized solid fermentation medium formula was wheat bran 59.5%, corn flour 35.0%, glucose 2.0%, lactose 1.0%, peptone 1.5%, and yeast extract 1.0%. The number of viable cells of L. plantarum reached lO0.5×10^8 CFU/g.
出处
《湖北农业科学》
北大核心
2012年第13期2818-2820,共3页
Hubei Agricultural Sciences
基金
武汉市科技攻关项目(201120637175-4)
