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复合处理钛合金表面对细胞生物相容性的影响 被引量:2

Effects of titanium alloy by composite treatment on cyto-biocompatibility of MG63 cells
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摘要 目的观察经磨砂、酸蚀、碱热复合处理的钛合金(Ti)表面对成骨样细胞(MG63细胞)生物相容性的影响。方法以2×104或1×105/孔将MG63细胞分别接种于A组(光滑纯n表面),B组(磨砂、酸蚀处理表面)和C组(磨砂、酸蚀、碱热复合处理表面)。对3组试样的MG63细胞的贴壁率、增殖率、碱性磷酸酶(AIJP)活性进行检测,用扫描电镜(SEM)对共培养后试样表面MG63细胞的形态进行观察。结果MG63细胞在A、B、C组的增殖率[吸光度(A)值]分别为0.752±0.080、0.614±0.020、0.567±0.062,A组与B、C组比较,差异有统计学意义(P〈0.05);A、B、C组的ALP活性分别为0.450±0.014、0.465±0.012、0.510±0.036,C组与A组比较,差异有统计学意义(P〈0.05);C组的细胞贴壁率高于A、B组(P〈0.05);SEM可见C组试样表面MG63细胞贴附、伸展状态好于A、B组。结论经磨砂、酸蚀、碱热复合处理的Ti表面对MG63细胞有良好的生物相容性,具有良好的细胞生物活性。 Objective To study effects of titanium alloy (Ti) by composite treatment using sand- blasting acid etehing (SLA) and alkali-heat (AH) on cyto-biocompatibility of MG63 cells. Methods Oste- oblast-like MG63 cells were cultured at 2 × 104 or 1 × 105 cells per well on samples of group A (pure Ti), group B (SLA Ti) and group C (SLA-AH Ti). The outcomes of cell adherence rate, cell proliferation rate, alkaline phosphatase (ALP) special assay were evaluated and compared and cell morphology was observed by scanning election microscopy (SEM). Results In group A, group B and group C, the cell proliferation rate was 0. 752 ±0. 080, 0. 614 ±0. 020 and 0. 567 ±0. 062, respectively (P 〈0. 05, group A vs group B or group C), the ALP special assay was 0. 450 ±0. 014, 0. 465 ±0. 012 and 0. 510 ±0. 036, respectively (P 〈0. 05, group C vs group A). The cell adherence rate in group C showed higher than in the two other groups (P 〈 0. 05 ), and the cells on composite treated Ti were attached weI1 and spread more extensively. Conclusion The surface of composite treated Ti is biocompatible and better bioactive than that of pure Ti or SLA Ti.
出处 《中华实验外科杂志》 CAS CSCD 北大核心 2012年第7期1257-1258,共2页 Chinese Journal of Experimental Surgery
基金 浙江省自然科学基金资助项目(Y2080956) 温州市科技计划资助项目(Y20070093)
关键词 钛合金 MG63细胞 生物相容性 Titanium Alloy MG63 cell Biocompatibility
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  • 1Tache A,Gan L, Deporter D, et al. Effect of surface chemistry on the rate of osseointegration of sintered porous-surfaced Ti-6Al-4V implants. Int J Oral Maxillofae Implants,2004,19 : 19-29.
  • 2Moritz N. Local induction of calcium phosphate formation on TiO2 coatings on titanium via surface treatment with a CO2 laser. J Biomed Mater Res,2003,65:9-16.
  • 3Pilliar RM. Dental implants: materials and design. J Can Dent Assoc,1990,56:857-861.
  • 4Qiu Q,Vincent P, Lowenberg B, et al. Bone growth on sol-gel calcium phosphate thin films in vitro. Cells and Materials, 1993,3:351-360.
  • 5Cook SD,Baffes GC, Palafox AJ, et al. Tortional Stability of HA-coated and Grit-blasted Titanium Dental Implants. J Oral implantol, 1992,28:359-356.
  • 6Orr RD, De Bruijn JD, Davie S. Scanning electron microscopy of the bone interface with titanium, titanium alloy and hydroxyapatite. Cells Materials, 1992,2 : 241-251.
  • 7Frank RM, Klewansky P, Hemmerle J, et al. Ultrastructural demonstration of the importance of crystal size of bioceramic powders implanted into human periodontal lesions. J Clin Periodontol, 1991,18:669-680.
  • 8Hench LL,Splinter RJ ,Allen WC,et al. Bonding mechanisms at the interface of ceramics prosthetic materials. J Biomed Mater Res, 1972,2 :117-141.
  • 9Chen CC, Huang TH, Kao CT, et al. Characterization of functionally graded hydroxyapatite/titanium composite coatings plasma-sprayed on Ti alloys. J Biomed Mater Res B Appl Biomater, 2006; 78(1):146-152
  • 10Lee BH, Kim JK, Kim YD, et al. In vivo behavior and mechanical stability of surface-modified titanium implants by plasma spray coating and chemical treat ments. J Biomed Mater Res A, 2004, 69(2) :279-285

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  • 1李鹏翠,卫小春.骨髓间充质干细胞的分离培养及体外分化[J].生物骨科材料与临床研究,2005,2(4):34-36. 被引量:3
  • 2杜瑞林,曾绍先,谢鑫荟.钛合金表面新型生物玻璃/纳米羟基磷灰石涂层的细胞相容性评价[J].生物骨科材料与临床研究,2007,4(5):6-9. 被引量:2
  • 3Mor GK,Varghese OK,Paulose M. A review on highly ordered,vertically oriented TiO2 nanotube arrays:Fabrication,material properties,and solar energy applications[J].{H}Solar Energy Materials and Solar Cells,2006,(14):2011-2075.
  • 4Bjursten LM,Rasmusson L,Oh S. Titanium dioxide nanotubes enhance bone bonding in vivo[J].{H}Journal of Biomedical Materials Research Part A,2010,(03):1218-1224.
  • 5Nagata M,Lonnerdal B. Role of zinc in cellular zinc trafficking and mineralization in a murine osteoblast-like cell line[J].{H}Journal of Nutritional Biochemistry,2011,(02):172-178.
  • 6Oh S,Brammer KS,Li YS. Stem cell fate dictated solely by altered nanotube dimension[J].{H}Proceedings of the National Academy of Sciences(USA),2009,(07):2130-2135.
  • 7Park J,Bauer S,vonder Mark K. Nanosize and vitality:TiO2 nanotube diameter directs cell fate[J].{H}Nano Letters,2007,(06):1686-1691.
  • 8Wang N,Li HY,Lu WL. Effects ofTiO2 nanotubes with different diameters on gene expression and osseointegration of implants in minipigs[J].{H}BIOMATERIALS,2011,(29):6900-6911.
  • 9Wang W,Zhao L,Ma Q. The role of the Wnt/beta-catenin pathway in the effect of implant topography on MG63 differentiation[J].{H}BIOMATERIALS,2012,(32):7993-8002.
  • 10Palacios C. The role of nutrients in bone health,from A to Z[J].{H}Critical Reviews in Food Science and Nutrition,2006,(08):621-628.

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