摘要
目的观察经磨砂、酸蚀、碱热复合处理的钛合金(Ti)表面对成骨样细胞(MG63细胞)生物相容性的影响。方法以2×104或1×105/孔将MG63细胞分别接种于A组(光滑纯n表面),B组(磨砂、酸蚀处理表面)和C组(磨砂、酸蚀、碱热复合处理表面)。对3组试样的MG63细胞的贴壁率、增殖率、碱性磷酸酶(AIJP)活性进行检测,用扫描电镜(SEM)对共培养后试样表面MG63细胞的形态进行观察。结果MG63细胞在A、B、C组的增殖率[吸光度(A)值]分别为0.752±0.080、0.614±0.020、0.567±0.062,A组与B、C组比较,差异有统计学意义(P〈0.05);A、B、C组的ALP活性分别为0.450±0.014、0.465±0.012、0.510±0.036,C组与A组比较,差异有统计学意义(P〈0.05);C组的细胞贴壁率高于A、B组(P〈0.05);SEM可见C组试样表面MG63细胞贴附、伸展状态好于A、B组。结论经磨砂、酸蚀、碱热复合处理的Ti表面对MG63细胞有良好的生物相容性,具有良好的细胞生物活性。
Objective To study effects of titanium alloy (Ti) by composite treatment using sand- blasting acid etehing (SLA) and alkali-heat (AH) on cyto-biocompatibility of MG63 cells. Methods Oste- oblast-like MG63 cells were cultured at 2 × 104 or 1 × 105 cells per well on samples of group A (pure Ti), group B (SLA Ti) and group C (SLA-AH Ti). The outcomes of cell adherence rate, cell proliferation rate, alkaline phosphatase (ALP) special assay were evaluated and compared and cell morphology was observed by scanning election microscopy (SEM). Results In group A, group B and group C, the cell proliferation rate was 0. 752 ±0. 080, 0. 614 ±0. 020 and 0. 567 ±0. 062, respectively (P 〈0. 05, group A vs group B or group C), the ALP special assay was 0. 450 ±0. 014, 0. 465 ±0. 012 and 0. 510 ±0. 036, respectively (P 〈0. 05, group C vs group A). The cell adherence rate in group C showed higher than in the two other groups (P 〈 0. 05 ), and the cells on composite treated Ti were attached weI1 and spread more extensively. Conclusion The surface of composite treated Ti is biocompatible and better bioactive than that of pure Ti or SLA Ti.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2012年第7期1257-1258,共2页
Chinese Journal of Experimental Surgery
基金
浙江省自然科学基金资助项目(Y2080956)
温州市科技计划资助项目(Y20070093)