摘要
目的探讨海尔福口服液对铝致老年痴呆症(AD)小鼠的治疗效果。方法建立铝致AD小鼠动物模型,将48只小鼠分为正常组、模型组、治疗1组(大剂量组)和治疗2组(小剂量组)。除正常组外,各组均进行铝染毒,用氯化铝水溶液拌饲料喂养和硝酸铝水溶液自由饮用,连续3个月。治疗1、2组在铝染毒2个月后同时给予海尔福口服液不同剂量灌胃,模型组和正常组用等量体积蒸馏水灌胃,直至实验结束。在实验前后分别测定血红蛋白(Hb)含量和进行水迷宫游水试验;实验后取血,测定血清中总钙、总胆固醇(TC)、甘油三酯(TG)、尿素的含量;处死小鼠取脑,称脑重量,取部分脑制成脑匀浆,离心取上清液,分别测定乙酰胆碱酯酶(AchE)、总巯基(-SH)、丙二醛(MDA)和超氧阴离子自由基(O2.-)清除率;另取部分脑用甲醛处理后作病理检查。结果 4个实验组的各种指标显示,AchE分别为(1.67±0.36)、(0.76±0.16)、(1.60±0.35)、(1.47±0.23)U/mg pro;-SH分别为(100.00±23.78)、(90.36±14.14)、(108.71±23.80)、(128.79±13.44)mmol/mg pro,与正常组比较,模型组偏低,差异有统计学意义(P<0.01);O2.-清除率分别为(17.63±3.05)%、(5.93±2.69)%、(16.20±2.36)%、(12.11±2.90)%,与正常组比较,模型组和治疗2组偏低,差异有统计学意义(P<0.01);大脑皮层病理检查显示,模型组有明显颗粒细胞数量减少,神经元萎缩、变性和凋亡现象。结论铝可致大脑神经元萎缩、变性和凋亡,导致AchE活力下降,抗氧化能力降低;中药海尔福通过排铝治疗对老年痴呆症有明显疗效。
[Abstract] Objective To explore the therapeutic effect of Haierfu on mice of aluminum induced Alzheimer＇s disease（AD）. Methods AD mice model was established first. 48 mice were divided into control group, model group, treatment group 1（large dose）and treatment group 2（small dose）. All mice except those of the control group were induced with aluminum by feeding aluminum chloride mixed fodder and freely drinking aluminum nitrate solution for 3 months. After two months, different doses of Haierfu were given to mice of treatment group 1 and group 2. Same volume of distilled water was given to mice of the control group and model group till to the end of the experiment. Hemoglobin（Hb） and Morris water maze test were tested before and after experiment. At the end of experiment total calcium, total eholesterol（TC）, triglyceride（TG） and urea in blood were determined. After mice were sacrificed, their brains were taken and weighed. Parts of the brain were made into homogenate, and centrifuged to get the supernatant, in which aeetylcholinesterase （AchE）, total sulfhydryl group （-SH）, malondialdehyde （MDA） and superoxide anion radical （O2^-） were determined. And another part of t＇he brain was used for pathological examination after formaldehyde treatment. Results In all experimental groups the indices showed that AchE was（1.67 ± 0.36）, （0.76 ± 0.16）, （1.60±0.35）, and（1.47±0.23）U/mgpro; -SHwas（100.00±23.78）, （90.36±14.14）, （108.71 ±23.80）, and（128.79±13.44）mmol/mg pro. The index in model group was a bit lower compared with the control group ; the difference was statistically significant （ P 〈 0. 01）. was（17.63± 3.05）%, （5.93±2.69）%, （16.20 ± 2.36）%, and（12.11± 2.90）%. The index in model group and treatment group 2 was a bit lower compared with the control group the differencewas statistically significant （P 〈0.01）. The pathological finding of pallium showed that the number of granular cell decreased obviously, and the neuron suffered from atrophy, degeneration and necrosis. Conclusions Aluminum can induce the brain neuron cell suffering from atrophy, degeneration and necrosis, which will reduce the AchE activity and the anti-oxidative ability. Chinese traditional medicine Haierful has obvious curative effect on AD through excreting aluminum.
出处
《工业卫生与职业病》
CAS
CSCD
北大核心
2012年第4期224-227,共4页
Industrial Health and Occupational Diseases
基金
广西自然科学基金项目(桂科0499013)
关键词
铝
老年痴呆症
乙酰胆碱酯酶
抗氧化能力
Aluminum
Alzheimer＇s disease
Acetyleholinesterase
Antioxidant ability
