摘要
目的:观察趋化因子白介素-8(Interleukin-8,IL-8)基因单核苷酸多态性与急性冠脉综合征(acute coronarysyndrome,ACS)的相关性。方法:采用直接测序的方法对675例ACS的患者和636例对照组进行检测,分析IL-8基因+394T/G单核苷酸多态的基因型和等位基因频率的分布情况。结果:IL-8基因+394 T/G单核苷酸多态在ACS组和对照组间的分布频率皆符合Hardy-Weinberg平衡定律,IL-8基因+394 T/G单核苷酸多态三种基因型(GG型,GT型和TT型)在ACS组分布频率分别为16.9%,48.7%和34.4%,在对照组分别为18.1%,50.9%和31.0%。IL-8基因+394 T/G多态基因型和等位基因频率在正常对照组和ACS组之间无明显的相关(P>0.05)。Logistic回归校正性别、年龄、体质量指数、吸烟、高血压病、高脂血症、糖尿病等冠心病易患因素后,IL-8基因+394T/G多态与ACS的发病无相关关系。结论:在中国北方汉族人群中IL-8基因+394T/G多态与ACS发病无相关关系,IL-8基因+394 T/G多态不是ACS发病的独立危险因素。
AIM: To investigate the association between IL-8 + 394T/G polymorphism and acute coronary syndrome (ACS) in a Hart population in Northern China. METHODS: A case-control study was conducted in 675 patients with ACS and 636 controls with normal coronary angiograms. Polymorphic genotypes were determined by polymerase chain reaction and sequencing analysis. RESULTS: Genotype frequencies in IL-8 + 394 T/G polymorphism were in good concordance with the Hardy-Weinberg equilibrium in both case and control groups. Genotype frequencies of GG, GT and TT of IL-8 + 394 T/G polymorphism were 18.1%, 50. 9% and 31.0% in the controls, and 16. 9%, 48.7% and 34.4% in patients with ACS, respectively. There were no significant differences in the genotype and allele distribution of + 394 T/G polymorphism of the IL-8 gene between groups (P 〉 0. 05 ). Logistic regression analysis with adjustments for other risk factors revealed that the IL-8 + 394 T/G allele carriers did not significantly increase the risks of ACS compared with noncarriers (P 〉 0. 05 ). CONCLUSIONS : IL-8 + 394 T/G polymorphism was not a genetic risk factor for ACS in a Han population in Northern China.
出处
《心脏杂志》
CAS
2012年第4期442-445,共4页
Chinese Heart Journal
基金
军队临床高新技术重大项目资助(2010GXJS001)
国家自然科学基金青年科学基金项目资助(81100135)