摘要
本试验通过对抗菌肽结构改造优化,得到了一条新设计的抗菌肽AMP-D,并利用固相合成技术人工合成后对其活性和溶血性进行测定。试验结果表明,AMP-D对革兰氏阴性菌及真菌没有生物活性,对革兰氏阳性菌有明显的抑制作用,且其没有溶血活性;AMP-D对金黄色葡萄球菌的最小抑菌浓度为10.5μg/mL,较原设计肽活性有较大提高。
By the experiment of antimicrobial peptides structure optimization and solid-phase synthesis technology of syn- thetic, new antibacterial peptide AMP-D could be obtained. According to determination of its activity and hemolytic, the experi- mental results showed that AMP D could inhibit the growth o[ Gram-positive bacteria, and was no hemolytic activity. Minimal inhibitory concentration of AMP D was 10.5 μg/mL against Staphylococcus aurous. The antimicrobial activities of AMP-D had more improve than the original peptide.
出处
《中国畜牧兽医》
CAS
北大核心
2012年第7期159-162,共4页
China Animal Husbandry & Veterinary Medicine
基金
广东食品药品职业学院院级资助项目(2009YZ003)
