摘要
目的探讨黄芪多糖对大鼠急性颈脊髓损伤后肺水肿的影响。方法成年Wist．ar大鼠200只，体重240-250g，雌雄不限。大鼠按随机数字表法分为五组：黄芪多糖组、低浓度黄芪多糖组、等渗盐水组、空白对照组、假手术组，每组40只。采用C，段颈脊髓改良Allen打击法制作大鼠颈脊髓损伤模型，打击力度为10×2．5g·cm；假手术组仅暴露C，段颈脊髓。各组按取材时间不同又分为造模后24h、3d、1周、2周共4个时相点，每个时相点10只大鼠。在不同时相点处死大鼠，通过光学显微镜观察肺组织的病理学变化，并测量各时相点每组大鼠肺湿重与干重（W／D）比值及血清和肺泡灌洗液蛋白浓度，计算肺W／D及肺通透性指数（LPI）。结果同一时相点低浓度黄芪多糖组和黄芪多糖组肺W／D比值及LPI均较空白对照组及等渗盐水组低，黄芪多糖组伤后3d时最低（P〈0．05）。结论大鼠急性颈脊髓损伤可造成肺组织水肿，伤后早期使用黄芪多糖可有效减轻颈脊髓损伤后肺水肿的程度。
Objective To investigate the effects of astragalin on pulmonary edema after acute cervical spinal cord injury in rats. Methods A total of 200 adult Wistar rats weighing 240-250 g were randomly divided into five groups： astragalin group, low concentration astragalin group, physiological sa- line group, blank group and sham group, with 40 rats in each group. The rats with cervical spinal cord injury were induced at C7 by modified Allen＇ s method, with the dropping weight of 10×2.5 g . cm. In the sham group, the laminas were removed only, leaving spinal cord at the C7 intact. Each group was fur- ther divided into four time points： 24 hours, 3 days, 1 week and 2 weeks after the modeling, with 10 rats in each time point, according to the specimen collection time. Rats were sacrificed at different time points to observe the pathological change of the lung tissue using optical microscope, measure the lung wet/dry weight ratio （W/D） and protein concentrations of the serum and bronchoalveolar lavage fluid and calcu- late the lung W/D and lung permeability index （LPI）. Results At the same instant, the W/D and LPI in the astragalin group and low concentration astragalin group were lower than those in physiological saline group and blank group, with the lowest value in the astragalin group at day 3 after injury （ P 〈 0.05 ）. Conclusions Rats with acute cervical spinal cord injury may cause pulmonary edema, which can be efficiently alleviated through early use of astragalin.
出处
《中华创伤杂志》
CAS
CSCD
北大核心
2012年第8期754-757,共4页
Chinese Journal of Trauma
基金
上海市科学技术委员会自然科学基金资助项目（11ZR1448400）
上海市教育委员会科研创新重点资助项目（12ZZ079）
关键词
脊髓损伤
肺水肿
黄芪多糖
Spinal cord injuries
Pulmonary edema
Astragalin
