摘要
目的探讨髓鞘碱性蛋白(MBP)免疫颅脑照射动物可否修复放射性脑损伤所致学习记忆能力下降。方法30只雄性SD大鼠随机分为3组,空白组、单纯照射组、照射加MBP免疫组,照射采用直线加速器,6MV-X线全脑照射,单次照射7 Gy,Morris水迷宫行为学检测,并进行免疫荧光组织化学及激光共聚焦显微技术计数新生神经元(BrdU+/DCX+)数量。结果照射加MBP免疫组Morris水迷宫行为学检测及新生神经元计数接近于空白组,差异无统计学意义(P>0.05);单纯照射组Morris水迷宫行为学检测及新生神经元计数明显少于空白组及照射+MBP免疫组,差异具有统计学意义(P<0.05)。结论髓鞘碱性蛋白可促进颅脑照射动物模型海马区神经元新生,可改善学习及记忆能力。
Objective To explore whether myelinbasicprotein may revert cognitive deficits of cranial irradiation injury. Methods Thirty SD rats were randomly divided into three groups ： the control group/irradiation group/irradiation＋MBP group. An- imals were exposed to cranial irradiation with a single dose of 7Gy. One month after vaccinated with MBP, Morris water waze studies were done. Immunofluorescence and confocal laser microscopy were used to estimate the number of double-positive cells. Results The behaviour tests and the number of double-positive ceils of irradiation group are longer and smaller than those of control and irra- diation＋MBP respectively, which vary significantly （P〈0.05）. Conclusion Myelinbasicprotein may repair cognitive deficits of cra- nial irradiation injury by inducing hippocampal neurogenesis.
出处
《解剖学研究》
CAS
2012年第3期173-175,共3页
Anatomy Research
基金
广州市医药卫生科技项目(201102A213119)
