摘要
制备了水溶性大豆多糖-铁(Ⅲ)配合物,通过单因素试验和响应面试验建立数学模型来对制备工艺进行优化,并采用紫外-可见光谱和傅里叶红外光谱对产物结构进行了鉴定.结果表明:在最佳工艺条件,即反应时间1.47 h、温度69.8℃、pH8.89、水溶性大豆多糖/柠檬酸三钠质量比1.92∶1下,含铁量的模型预测值为31.94%,而对应的实测值为30.65%,说明所建立的水溶性大豆多糖-铁(Ⅲ)响应面模型可以用于指导大豆多糖-铁(Ⅲ)的制备;水溶性大豆多糖(SSPS)与铁(Ⅲ)发生了配合反应,铁以β-FeOOH结构聚合成铁核,SSPS以羧基与铁核配合后得到水溶性大豆多糖-铁(Ⅲ)配合物.
In this paper,soluble soybean polysaccharide（SSPS）-Fe（Ⅲ） complex was prepared,and a mathemati-cal model based on the single factor test and the response surface test was established to optimize the corresponding preparation conditions.Then,the structure of the complex was identified by means of UV-Vis and FT-IR.The results show that,under the optimal conditions,namely a reaction time of 1.47 h,a temperature of 69.8 ℃,a pH value of 8.89 and a mass ratio of SSPS to trisodium citrate of 1.92 ∶ 1,the tested Fe（Ⅲ） content of the complex,namely 30.65%,is close to the predicated value of 31.94%,which means that the proposed model based on the response surface methodology is reasonable.Moreover,it is found that SSPS-Fe（Ⅲ） complex can be successfully prepared by the reaction of the carboxyls in SSPS with the iron core formed through β-FeOOH structure aggregation.
出处
《华南理工大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
2012年第5期127-132,144,共7页
Journal of South China University of Technology(Natural Science Edition)
基金
广东省科技计划项目(2010A080403005)
广州市重大科技专项(2010U1-D00021)
华南理工大学中央高校基本科研业务费专项资金资助项目(2012ZM0072)
关键词
水溶性大豆多糖
铁(Ⅲ)
配合物
制备
响应面分析法
工艺优化
结构表征
soluble soybean polysaccharide
Fe （ Ⅲ）
complex
preparation
response surface methodology
process optimization
structural characterization
