摘要
背景:尿酸盐阴离子交换器在调节全身的尿酸盐稳态中起重要作用,Ki-67是细胞增殖的重要标志物,那么二者对高尿酸血症大鼠主动脉平滑肌细胞有何影响呢?目的:观察高尿酸血症对大鼠主动脉平滑肌细胞Ki-67及尿酸盐阴离子交换器表达的影响。方法:20只8周龄雄性Wistar大鼠随机摸球法均分为实验组和对照组。实验组给予高酵母饲料喂养,同时给予腺嘌呤溶液灌胃,对照组给予普通饮食及同体积蒸馏水灌胃。当血尿酸水平出现差异时,继续饲养5周处死所有大鼠,苏木精伊红染色观察主动脉平滑肌组织结构,免疫组化检测胸主动脉平滑肌细胞增殖核抗原Ki-67以及尿酸盐阴离子交换器的表达。结果与结论:罹患5周高尿酸血症对16周龄Wistar大鼠主动脉平滑肌细胞Ki-67及尿酸盐阴离子交换器表达差异无显著性意义,也未造成动脉粥样硬化样改变。说明高尿酸血症作为动脉硬化的影响因素之一,可能需要更长期的病程才可能体现出来。
BACKGROUND： Urate anion exchanger （URAT1） plays an important role in adjusting steady-state of urate in the whole body, and ki-67 is an important cell multiplication marker. What is their effect on the aortic smooth muscle cells （ASMCs） of hyperuricemia rats？ OBJECTIVE： To observe the effect of hyperuricemia on the expression of Ki-67 in ASMCs and URAT1. METHODS： Twenty 8-week-old Wistar rats were randomly divided into two groups： control group （n=10） and experimenta group （n=10）. Rats in the experimental group were fed with high yeast and gavaged with amidopurine fluid. However, rats in the control group were fed with general diet and gavaged with the same dose of distilled water. When the level of blood uric acid started to show the difference, all the rats were fed for another 5 weeks and then were sacrificed. Hematoxylin-eosin staining was used to observe the structure of aortic smooth muscle and immunohistochemistry was used to detect the expression of Ki-67 in ASMCs and URATI. RESULTS AND CONCLUSION： Hyperuricemia that persisted for 5 weeks had no obvious effect on the expression of Ki-67 and in ASMCs and URAT1 in 16-week-old male Wistar rats, or did not result in atheroscterosis. As an influential factor of atherosclerosis, hyperuricemia needs more time to result in atherosclerosis.
出处
《中国组织工程研究》
CAS
CSCD
2012年第28期5222-5226,共5页
Chinese Journal of Tissue Engineering Research
