摘要
目的:探索评价基质效应在化学发光免疫分析中对甲状腺功能五项指标的影响。方法:选取甲状腺功能五项高值血清,用10种基质牛血清、马血清、山羊血清、水解明胶、BSA、PBS、生理盐水、正常人血清、甲减人血清、甲亢人血清分别对T3、T4、FT3、FT4、TSH的高值血清进行倍比稀释,观察基质效应,另将10种基质用考马斯亮兰法检测蛋白含量,分析蛋白含量与基质效应的关系。结果:T3项目牛血清、水解明胶、BSA有明显基质效应;T4和FT3项目牛血清、水解明胶、BSA、PBS、生理盐水有明显基质效应;FT4项目牛血清、马血清、水解明胶、BSA、PBS、生理盐水有明显基质效应;TSH项目没有发现基质效应,正常人血清、甲减人血清和甲亢人血清对甲状腺功能五项无基质效应。检测结果显示蛋白含量多少与基质效应无关。结论:人血清基质是用于稀释样本,基质效应最小的液体,针对个体差异性进行的选择,稀释T3、T4、FT3、FT4高值血清选择甲减人血清,稀释TSH高值血清选择甲亢人血清,可以得到较为满意的结果。
Objective： To evaluate clinical test interference by matrix effect of five analytes of thyroid using chemiluminescent immunoassay.Methods： Collected high value serum of five analytes of thyroid.In order to test matrix effect,the high value serum of T3,T4,FT3,FT4,TSH were diluted by bovine serum,horse serum,goat serum,hydrolyzed gelatin,BSA,PBS,physiological saline solution,normal human serum,hyperthyroidism human serum and hypothyroidism human serum.Compared protein concentration of the ten matrix for finding relationship between protein concentration and matrix effect.Results： Bovine serum,hydrolyzed gelatin and BSA made matrix effect in T3 assay;Bovine serum,hydrolyzed gelatin,BSA,PBS and physiological saline solution made matrix effect in T4 and FT3 assay;Bovine serum,horse serum,hydrolyzed gelatin,BSA,PBS and physiological saline solution made matrix effect in FT4 assay;None of 9 matrix made matrix effect in TSH assay.There was no matrix effect to use normal,hyperthyroidism and hypothyroidism human serum for the five analytes of thyroid.There was no relationship between protein concentration and matrix effect.Conclusions： Human serum was the minimum effect of the total matrix,it was needed to select suitable solution for different individual sample for getting good result.High valve sample of T3,T4,FT3,FT4 selected hypothyroidism human serum to dilute;High valve sample of TSH selected hyperthyroidism human serum to dilute.
出处
《现代生物医学进展》
CAS
2012年第19期3714-3718,3713,共6页
Progress in Modern Biomedicine
关键词
基质效应
化学发光免疫分析
甲功五项
Matrix Effect
Chemiluminescent Immunoassay
Five Analytes of Thyroid
