摘要
目的:检测急性乙醇中毒大鼠经葛根素干预后多脑区(皮层、小脑、海马和纹状体)超氧化物歧化酶(SOD)、丙二醛(MDA)及谷氨酸(Glu)的含量,观察大鼠的行为学变化,探讨葛根素对急性乙醇中毒大鼠的干预作用。方法:雄性SD大鼠随机分为对照组、乙醇中毒组(高、中、低剂量组)和葛根素干预组(干预中剂量乙醇中毒),检测各组大鼠皮层、小脑、海马和纹状体内SOD、MDA、Glu含量和转棒实验。结果:与对照组比较,乙醇中毒组大鼠各脑区SOD活性和Glu含量降低,MDA含量升高(P<0.05或P<0.01),且SOD和MDA的变化具有剂量依赖性,大鼠棒上停留时间缩短(P<0.01);与乙醇中毒组比较,葛根素干预组大鼠各脑区SOD活性和Glu含量升高,MDA降低(P<0.05或P<0.01),其中海马的变化幅度最大,大鼠棒上停留时间延长(P<0.05或P<0.01)。结论:葛根素对急性乙醇中毒大鼠脑氧自由基和Glu有干预作用,对乙醇中毒大鼠行为学有调控作用。
Objective To investigate the interference effects of puerarin on multitude brain regions,which contains cortex,cerebellum,hippocampus and striatum of acute alcoholism rats,by detecting the contents of superoxide dismutase（SOD）,malondialdehyde（MDA） and glutamate（Glu） in rat brain and the praxiological changes after administration with ethanol and puerarin.Methods Male SD rats were randomly divided into control group,alcoholism groups（high dose group,middle dose group and low dose group）,and puerarin group（the rats were treated with middle dose alcoholism）.The contents of SOD,MDA,Glu and the rodduration were detected.Results Compared with control group,the contents of SOD and Glu in alcoholism groups were reduced,while the MDA was increased.The contents of SOD and MDA changed in dose-dependent manner.The rodduration in alcoholism groups was significantly shorter than that in control group（P〈0.05 or P〈0.01）.Compared with alcoholism groups,the rodduration in puerarin group became longer,the contents of SOD and Glu were increased,the content of MDA was reduced（P〈0.05 or P〈0.01）,and the most significant changes were found at hippocampus among the four brain regions.Conclusion Puerarin has interference effects on oxygen free radical and Glu in brain of acute alcoholism rats,and it exhibites a regulatory effect on praxiology of acute alcoholism rats.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2012年第4期665-668,共4页
Journal of Jilin University：Medicine Edition
基金
山东省科技厅自然科学基金资助课题(ZR2010CL018)
山东省德州市科技局科技计划基金资助课题(20090162-4)
关键词
急性乙醇中毒
多脑区
氧自由基
谷氨酸
行为学
葛根素
acute alcoholism
multitude brain regions
oxygen free radical
glutamate
praxiology
puerarin
