摘要
目的调查云南省大理州不明原因发热患者是否存在斑点热。方法用巢式-聚合酶链反应法扩增患者血液中的立克次体属groEL基因,并进行核酸序列鉴定和分析。结果 20例患者血液中检出1例立克次体核酸阳性(检出率5%),其序列分析与GenBank中Rickcttsia rickcttsii Iowa、Rickcttsia peacockii Rustic、Rickcttsia sibirica等斑点热群立克次体株的同源性最高(为99%)。结论首次证实云南大理地区存在斑点热患者,医疗部门应加强对斑点热的诊断与鉴别。
Objective To identify the pathogen of patients with fever with unknown origin and determine whether they were infected with spotted fever group Rickettsia. Methods Nested polymerase chain reaction （nPCR） was conducted to amplify groEL gene of rickettsia from the blood samples of the patients, the nucleotide sequencing and identification were carried out. Results Rickettsial DNA was detected in 1 of the 20 patients （5%）. The groEL gene of the strain shared high homology （99%） with Rickcttsia rickcttsii Iowa, Rickcttsia peacockii Rustic and Rickcttsia sibirica. Conclusion It was the first time to diagnose the patient with spotted fever in Dali, Yunnan province. It is necessary to enhance the differential diagnosis of spotted fever in local medical institutions.
出处
《疾病监测》
CAS
2012年第6期432-434,共3页
Disease Surveillance
关键词
斑点热
斑点热群立克次体
不明原因发热
实验室检查
云南大理
spotted fever
spotted fever group Rickettsia
fever with unknown origin
laboratory detection
Dali, Yunnan
