摘要
目的:比较2种测量盐酸多西环素含量方法。方法:紫外分光光度法与高效液相色谱法。结果:紫外分光光度法测盐酸多西环素,在0.002～0.028 mg/mL范围内线性关系良好(r=0.9999),添加回收率为99.12%(n=5,RSD=1.79%);高效液相色谱法测盐酸多西环素,在0.005～0.180 mg/mL范围内线性关系良好(r=0.9999),添加回收率为99.90%(n=5,RSD=1.76%)。结论:紫外分光光度法操作方便快捷,高效液相色谱法更为可靠。
Objective： The research aimed to compare two different determination methods of doxycycline hydrochloride content. Method： The ultraviolet spectrophotometry method and HPLC method were used to determine doxycycline hydrochloride content. Result： The standard curve of ultraviolet spectrophotometry method showed a good linear relationship from 0.002 mg/mL to 2.8 mg/mL and the average recovery was 99.12 % （n=5, RSD=1.79 %）.The standard curve of HPLC method showed a good linear relationship from 0.005 mg/mL to 0.180 mg/mL and the average recovery was 99.90 % （n=5, RSD= 1.76 %）. Conclusion： The ultraviolet spectrophotometry method was more convenienct, the HPLC method was more reliable.
出处
《广东化工》
CAS
2012年第7期189-190,188,共3页
Guangdong Chemical Industry
