摘要
[目的]观察四逆汤预干预对高脂血症(HLP)合并动脉粥样硬化(AS)兔的TC、TG、HDL-CH、LDL-CH、Apo-A、Apo-B的影响,分析其对高脂血症(HLP)合并动脉粥样硬化(AS)兔血脂及载脂蛋白的调节作用。[方法]通过高脂饲料喂养及免疫损伤法建立兔HLP合并AS模型,分别给予西药(阿托伐他汀)和四逆汤(高、中、低剂量)的治疗方法。运用酶法测定血中TC、TG含量的变化;比色法测定干预后血中LDL-CH、HDL-CH含量的变化;电泳法测干预后血中载脂蛋白A及B含量的变化。[结果]四逆汤中、高剂量组在降低血TG含量与模型组比具有统计学意义(P<0.05);四逆汤高剂量组在降低血TC、LDL、Apo-B含量与模型组比具有统计学意义(P<0.05);在升高血HDL、Apo-A含量,HDL/LDL比值,Apo-A/Apo-B比值方面,四逆汤高剂量组与模型组比具有显著性差异(P<0.01)。[结论]四逆汤各剂量组均能调节实验性HLP并AS兔血脂代谢,降低胆固醇和甘油三酯的含量,且能降低血中低密度脂蛋白和载脂蛋白B的含量,使其高密度脂蛋白、载脂蛋白A升高。四逆汤预防AS形成的作用机制可能与调节血脂代谢,减缓脂质颗粒沉积有关。
[ Objective ] To observe the effect on TC ,TG,HDL.LDL,Apo - A,Apo - B of HLP combined AS rabbits treated by Sini Decoction, and to analyze the regulatingeffect on blood lipid and apolipoprotein of this therapy. [Method]While fed with high fat food everyday and injected with BSA, egg -white protein, HLP combined AS rabbits were treated with different way of Atorvastatin and three doses of Sini Decoction （ High, middle and low）. To observe the changes after the intervention of the use of enzymatic determination of serum TC, TG, NO level ; colorimetric assay after the intervention of blood LDL - CH, HDL - CH levels ; electrophoresis after the intervention measured blood levels of apolipoprotein A and B changes. [ Results ]It is has statistical meaning that the TG content of groups of high dose and middle dose Sini Decoction are lowered compared with model group （P 〈 0.05 ）. It is has statistical meaning that the TC. LDL and Apo - B content of group of high dose Sini Decoction are lowered compared with model group （ P 〈 0.05 ）. The HDL and Apo - A content of group of high dose Sini Decoction are obviously higher than that of model group, as well as the ratio of HDL/LDL and Apo - A/Apo - B. The difference between them has very remarkable meaning （ P 〈 0.01 ）. [ Conclusion] SNT can adjust the lipid metabolism of experimental HLP combined with AS in rabbit model, lower cholesterol and triglyceride levels and decreased serum LDL and Apo - B levels, so that HDL and Apo - A increased. SNT to prevent the formation mechanism of AS may be related to blood lipid metabolism, aortic endothelial damage, slow down the lipid granules.
出处
《实用中医内科杂志》
2012年第7期1-3,6,共4页
Journal of Practical Traditional Chinese Internal Medicine
基金
长沙市科技计划重点项目(k1005020-31)
湖南省教育厅项目(NO:06C629)
关键词
四逆汤
高脂血症
动脉粥样硬化
血脂及载脂蛋白
Sini Decoction
Hyperlipemia
Artherosclerosis
Blood lipid and apolipoprotein
