摘要
目的:探讨凋膜止崩液宫腔靶向给药治疗无排卵性功能失调性子宫出血(功血)子宫内膜增生症的可能机制。方法:选取健康成年SD雌性大鼠48只,分为空白组、模型对照组、实验小剂量组和实验大剂量组,建立无排卵性功血子宫内膜增生症模型。凋膜止崩液宫腔内给药后3d处死,留取子宫标本,采用免疫组化技术检测各组大鼠子宫内膜组织中雌激素受体(ER)、ERα及ERβ的表达水平。结果:模型对照组ER、ERα、ERβ的表达高于空白组,实验大、小剂量组ER、ERα、ERβ的表达低于模型对照组,差异均有统计学意义(均P<0.001),实验大剂量组ER、ERα和ERβ水平低于实验小剂量组,差异有统计学意义(P<0.01)。结论:凋膜止崩液通过下调ER、ERα及ERβ阻断了雌激素发挥效应的通路,抑制子宫内膜的持续性增生从而治疗子宫内膜增生症。
Objective： To explore the role and the possible mechanism ot Diaomozhibeng liquid in rats with the endome- trial hyperplasia （EH）. Methods： Forty-eight SD rats were divided into four groups including control group, model control group, the experimental low-dose group and the experimental high-dose group. The rat model of endometrial hyperplasia was induced with estrogen load. The Diaomozhibeng liquid was administrated in uterine cavity in rats. The expression levels of es- trogen receptor （ER）, ERα and ERβ were checked by immunohistochemistry. Results： Compared with control group, expres- sions of ER, ERa and ERβ increased in rats of the model group （P 〈 0.001）. However, after treatment with Diaomozhibeng liq- uid, the expression of ER, ERα and ERβ significantly decreased （P 〈 0.001）. Expression levels of ER, ERα and ERβ were significantly lower in high-dose experimental group than those of low-dose experimental group （P 〈 0.05 or P 〈 0.01）. Con- clusion： Diaomozhibeng liquid plays a role in endometrial hyperplasia by accelerating the ecclasis of uterine endometrial. One of mechanisms is maybe related to inhibiting the expression of ER, ERα and ERβ
出处
《天津医药》
CAS
北大核心
2012年第8期793-795,共3页
Tianjin Medical Journal
基金
国家自然科学基金资助项目(项目编号:30873354)
陕西省"13115"科技创新工程重大科技专项计划项目(项目编号:2008ZDKG-81)
关键词
功能性子宫出血
子宫内膜增生
受体
雌激素
雌激素受体α
雌激素受体Β
凋膜止崩液
tunctional uterine bleeding endometrial hyperplasia receptors, estrogen estrogen receptor alpha estro-gen receptor beta diaomozhibeng liquid
