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人疱疹病毒6型感染与神经胶质瘤关系的初步研究 被引量：3

Preliminary study on the relationship between human herpesvirus-6 infection and glioma
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摘要 目的:研究人疱疹病毒6型(human herpesvirus-6,HHV-6)感染与神经胶质瘤的关系。方法:巢式PCR法检测40例神经胶质瘤样本和13例正常脑组织样本中HHV-6、人巨细胞病毒(human cytomegalovirus,HCMV)、HHV-7 DNA序列。免疫组化染色法检测神经胶质瘤样本和正常脑组织样本中HHV-6、HCMV、HHV-7抗原的表达。结果:巢式PCR法检测结果显示,神经胶质瘤组织HHV-6 DNA阳性率为42.5%,正常脑组织HHV-6 DNA阳性率为7.7%(P=0.020),神经胶质瘤组织HCMV、HHV-7DNA阳性率分别为20.0%和5.0%,正常脑组织未检测到HCMV和HHV-7 DNA(P值分别为0.087和0.566)。进一步用免疫组化染色法检测HHV-6早期抗原p41的表达,神经胶质瘤组织阳性率为27.5%,正常脑组织未见阳性表达(P=0.030)。同时检测HHV-6晚期抗原gp116/64/54的表达,神经胶质瘤组织阳性率为32.5%,正常脑组织未见阳性表达(P=0.014)。用HCMV pp65抗体进行免疫组化染色后发现其在神经胶质瘤中阳性率为12.5%,正常脑组织未见阳性表达(P=0.229)。HHV-7 pp85抗原在神经胶质瘤样本和正常脑组织样本中均未检出阳性表达。结论:根据PCR和免疫组化的检测结果,HHV-6感染在神经胶质瘤和正常脑组织中具有显著性差异,HHV-6感染在神经胶质瘤的病因和发展过程中起了一定作用。 Objective： To study the relationship between human herpesvirus（HHV）-6 infection and glioma.Methods：HHV-6,human cytomegalovirus（HCMV） and HHV-7 DNA in glioma samples（n = 40） and control samples（n = 13） were detected by nested PCR.The expression of HHV-6,HCMV and HHV-7 was detected by immunohistochemistry（IHC）.Results：HHV-6 DNA was positive in 17/40（42.5%） of glioma samples and 1/13（7.7%） of normal brain samples（P = 0.020）,while the positive rates of HCMV and HHV-7 DNA were 20.0% and 5.0% in glioma,respectively.HCMV and HHV-7 DNA hadn’t been found in normal brain samples（P = 0.087 and 0.566 respectively）.HHV-6 late antigen p41 was expressed in 11/40（27.5%） of tumors and 0 of the controls（P = 0.030）,while HHV-6 early antigen gp116/64/54 was expressed in 13/40（32.5%） of tumors and 0 of the controls（P = 0.014）.HCMV pp65 antigen was expressed in 5/40（12.5%） of glioma samples and 0/13 of the normal brain controls（P = 0.229）.HHV-7 pp85 antigen hadn’t been found in tumors and normal brain samples.Conclusion：Based on the results of nested PCR and immunohistochemistry,significant difference of HHV-6 infection between glioma and normal brain tissue was confirmed.HHV-6 infection may play a role on the pathagenesis and progression of glioma.
作者 张春 茌静 顾斌 郭丹丹 张国锋 周锋 魏栋 姚堃 胡卫星
机构地区 南京医科大学第一附属医院神经外科 南京医科大学微生物与免疫学系
出处 《南京医科大学学报（自然科学版）》 CAS CSCD 北大核心 2012年第8期1052-1056,共5页 Journal of Nanjing Medical University(Natural Sciences)
基金 国家自然科学基金资助(30972784) 江苏省医学重点人才工程(135工程)资助(RC2002075)
关键词 人疱疹病毒6型 神经胶质瘤 巢式PCR 免疫组化 human herpesvirus-6； glioma； nested PCR； immunohistochemistry
分类号 R739.41 [医药卫生—肿瘤]
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参考文献20

  • 1Scheurer ME,Bondy ML,Aldape KD,et al. Detection of human eytomegalovirus in different histological types of gliomas[ J ]. Acta Neuropathol, 2008,116(1 ) : 79-86.
  • 2Cobbs CS,Harkins L,Samanta M,et al. Human cytome-galovirus infection and expression in human malignant glioma[J~. Cancer Res, 2002,62(12) : 3347-3350.
  • 3Poltermann S,Schlehofer B ,Steindorf K,et al. Lack of as- sociation of herpesviruses with brain tumors [J]. J Neu- rovirol,2006,12(2) :90-99.
  • 4Salahuddin SZ,Ablash DV, Markham PD, et aL Isolation of a new virus, HBL, in patients with lymphoprolifera- tive disorders[J]. Science, 1986,234(4776) : 596-601.
  • 5Caserta MT. Human herpesvirus 6 infection of the central nervous system [J]. Curr Infect Dis Rep,2004,6 (4): 316-321.
  • 6Yao K,Gagnon S,Akhyani N,et al. Reactivation of hu- man herpesvirus-6 in natalizumab treated multiple scle- rosis patients [ J ]. PLoS One, 2008,3 ( 4 ) : e2028.
  • 7Fotheringham J, Donati D,Akhyani N, et al. Association of human herpesvirus-6B with mesial temporal lobe epilepsy[J~. PLoS Med,2007,4(5) :elS0.
  • 8Fotheringham J, Akhyani N, Vortmeyer A, et al. Detection of active human herpesvirus-6 infection in the brain: correlation with polymerase chain reaction detection in cerebrospinal fluid [ J ]. J Infect Dis, 2007,195 (3) : 450- 454.
  • 9Crawford R,Santi R,Thorarinsdottir K,et al. Detection of human herpesvirus-6 variants in pediatric brain tumors: Association of viral antigen in low grade gliomas [J]. J ClinVirol,2009,46( 1 ) : 37-42.
  • 10Crawford R,Santi R,Cornelison R,et al. Detection of hu- man herpesvirus-6 in adult central nervous system tu- mors:predominance of early and late viral antigens in glial tumors [ J ]. J Neurooncol, 2009,95 ( 1 ) : 49-60.

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同被引文献35

  • 1Saddawi KR, Crawford JR. Chronic viral infection and primarycentral nervous system malignancy [ J ]. J Neuroimmune Pharma- col, 2010, 5(3) : 387-403.
  • 2Lacroix A, Collot TS, Mardivirin L, et al. Involvement of human herpesvirus-6 variant B in classic Hodgkin' s lymphoma via DR7 oncoprotein[J]. Clin Cancer Res, 2010, 16(19) : 4711-4721.
  • 3Crawford JR1, Santi MR, Cornelison R, et al. Detection of human herpesvirus-6 in adult central nervous system tumors: predomi- nance of early and late viral antigens in glial tumors[ J ]. J Neu- rooncol, 2009, 95 ( 1 ) : 4940.
  • 4Crawford JR, Santi MR, Thorarinsdottir HK, et al. Detection of human herpesvirus-6 variants in pediatric braintumors: association of viral antigen in low grade gliomas[ J]. J Clin Virol, 2009, 46 (1) : 37-42.
  • 5Salahuddin SZ, Ahlashi DV,Markham PD,et al. Isolationof a new virus, HBLV, in patients with lymphoproliferative disorders[J]. Science, 1986,234 (4776): 596-601.
  • 6Safronetz D,Humar A,Tipples GA. Differentiation and quantitation of human herpesviruses 6A,6B and 7 by real- time PCR [J]. J Virol Methods,2003,112(1-2):99-105.
  • 7Dominguez G,Dambaugh TR,Stamey FR,et al. Human herpesvirus 6B genome sequence:coding content and comparison with human herpesvirus 6A[J]. J Virol, 1999,73 (10) : 8040-8052.
  • 8Gompels UA,Nicholas J,Lawrence G,et al. The DNA se- quence of human herpesvirus-6:structure,coding content, and genome evolution[ J ]. Virology, 1995,209 ( 1 ) : 29-51.
  • 9Ablashi DV,Balachandran N,Josephs SF,et al. Genomic polymorphism, growth properties, and immunologic varia- tions in human herpesvirus-6 isolates[J]. Virology, 1991,184 (2) :545-552.
  • 10Yamanishi K, Okuno T, Shiraki K, et al. Identification of human herpesvirus-6 as a causal agent for exanthem subitum[J]. Lancet, 1988,1 (8594) : 1065-1067.

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南京医科大学学报（自然科学版）
2012年 第8期

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