摘要
目的:探讨清热解毒、活血化瘀法对大鼠原位肝移植急性排斥反应免疫调节的作用机制。方法:以Wistar、SD大鼠分别作为供、受体,建立大鼠原位肝移植急性排斥反应模型,并将其随机分为生理盐水、复方中药、环孢素A、复方中药加环孢素A等4组。各组受体大鼠术前第1天开始至术后1月分别灌胃给药,每日1次。生理盐水组仅给予生理盐水2mL/d,复方中药组给予复方中药2mL/d,环孢素A组给予环孢素A 10mg/(kg.d),复方中药加环孢素A组给予复方中药1mL/d+环孢素A 5mg/(kg.d)。各组受体于术后第1、3、5、7天分别取尾静脉血,经ELISA法检测白细胞介素-2(IL-2)、白细胞介素-10(IL-10)的含量。术后第7天,每组各处死10只大鼠,光镜下观察肝脏组织病理改变。每组余下大鼠观察生存期。结果:与生理盐水组比较:①复方中药、环孢素A、复方中药加环孢素A组大鼠的生存时间分别为(14.0±1.4)d,(18.4±1.5)d,(27.8±2.0)d(P<0.05);②在同一时间点,其他用药组大鼠外周血IL-2的值明显下降(P<0.05),复方中药组、复方中药加环孢素A组IL-10的值则有所上升(P<0.05),而环孢素A组IL-10的值无明显变化(P>0.05);③各用药组大鼠移植肝病理排斥反应程度明显降低,以复方中药加环孢素A组最为明显。结论:复方中药对大鼠原位肝移植急性排斥反应具有抑制作用,且与环孢素A具有协同作用,其作用机制可能与降低IL-2、上调IL-10的水平有关。
Objective ： To analyse the mechanism of the function of clearing away heat, detoxieating, promoting blood circulation and dissolving blood stasis on acute rejection after liver transplantation in rats. Method： Liver recipients were randomly divided into four groups：normal saline group, compound traditional Chinese medicine group, Cyelosporine A group and compound traditional Chinese medicine plus Cyclosporine A group. The recipients were administered intragastricly from operation eve to the next 30 days after the operation once a day. Normal saline group was only administered intragastriely with nornml saline at dose of 2mL/d. Compound traditional Chinese medicine group at dose of 2mL/d. C, yclosporine A group at dose of 10mg/（kg · d）. Compound traditional Chinese medicine plus Cyclosporine A group, compound traditional Chinese medicine was administered intragastricly lmL/d following Cyclosporine A 5mg/（kg · d）. The concentrations of IL-2 and IL-10 in the plasma were measured using ELISA on the first, third, fifth and seventh day after the operation in every group. Ten recipients from each group were sacrificed on the seventh day after the operation and their liver tissue was collected to observe the intensity of acute rejection. The rest recipients in each group were fed, and their survival time was recorded. Results： Compared with the Normal Saline Group： （1）The survival time of the other groups was longer （P〈0.05）. The exact survival time of Compound Traditional Chinese Medicine Group was （ 14.0 ± 1.4 ） d, while Cyelosporine A Group＇s was （ 18.4 ± 1.5 ） d, and Compound Traditional Chinese Medicine plus Cyclosporine A Group＇s was （27.8 ± 2.0 ）d.（2）At a same time point, the IL-2 levels of other groups＇ peripheral blood were obviously reduced （P〈0.05）, and the IL-10 level of Compound Traditional Chinese Medicine Groupand Compound Traditional Chinese Medicine plus Cyclosporine A Group raised a little （ P〈0.05 ） while Cyclosporine A Group＇s had no obvious change. （3）The severity of acute rejection was obviously decreased in the other groups （P〈0.05）, especially Compound Traditional Chinese Medicine plus Cyclosporine A group. Conclusion ： Compound traditional Chinese medicine can inhibit the severity of rat hepatic allograft acute rejection and has synergistic effect with Cyclosporine A. The effective mechanisms may concern with its effect on reducing IL-2 level and raising IL-10 level in blood.
出处
《辽宁中医药大学学报》
CAS
2012年第8期5-8,共4页
Journal of Liaoning University of Traditional Chinese Medicine
基金
江西省科技厅社会发展攻关计划项目(2002)
关键词
清热解毒活血化瘀法
大鼠
肝移植
急性排斥反应
免疫调节
function of clearing away heat, detoxicating, promoting blood circulationand dissolving blood stasis
rat
liver transplantation
acute rejection
immunoloregulation
