摘要
目的:建立肉苁蓉多糖的含量测定方法。方法:以精制肉苁蓉多糖测得肉苁蓉多糖对葡萄糖的换算因子,利用苯酚-硫酸法测定肉苁蓉多糖含量。结果:测得肉苁蓉生药中多糖含量12.0%,平均回收率为99.81%,RSD=2.67%(n=6)。结论:该方法简便,可行,可用于肉苁蓉多糖的含量测定。
Objective：The aim of the paper was to determine the content of polysaccharides in Herba Cistanche.Method：The conversion coefficient of Herba Cistanche polysaccharides to glucose was obtained by refined polysaccharides,and then the content of polysaccharides in crude polysaccharides fraction was determined by phenolsulfuric acid method.Result：The average concentration of polysaccharides was 12.0%,and the average recovery was 99.81%（n=6,RSD=2.67%）.Conclusion：The method used in this paper is simple.It can be used fordetermination of the polysaccharide in Herba Cistanche.
出处
《辽宁中医药大学学报》
CAS
2012年第8期100-101,共2页
Journal of Liaoning University of Traditional Chinese Medicine
基金
阿拉善盟科技局阿左旗科技计划项目(2011-19)
关键词
肉苁蓉多糖
苯酚-硫酸法
含量测定
Herba Cistanche polysaccharides； phenolsulfuric acid method； content determination；
