摘要
对广西绞股蓝种子进行了生活力测定、发芽试验、吸水率测定及离体胚萌发试验,并对广西绞股蓝种子进行酸碱处理、低温层积和干藏处理、GA_3和6-BA溶液处理,探讨不同的处理对广西绞股蓝种子萌发的影响。结果表明新鲜广西绞股蓝种子的活力高达98%,但在6个恒温和1个变温条件下,其萌发率最高仅为11.7%,说明广西绞股蓝具有明显的休眠现象。种子吸水性测定结果表明,广西绞股蓝种皮限制种子吸水,离体胚萌发实验证实广西绞股蓝种子的胚在离体条件下没有休眠现象,说明种皮障碍是导致广西绞股蓝种子休眠的主要原因。低温层积不能解除广西绞股蓝种子的休眠,而干藏则会导致种子内部失水,从而导致种子生活力降低。浓硫酸和不同浓度的NaOH溶液处理均不能打破种子的休眠,仅6-BA溶液处理对4℃层积后的种子萌发起到一定的促进作用。
Seed viability test, seed germination test, water-absorbing test, isolated embryos germination test of Gynostemma guangxiense X. X. Chen et D. H. Qin were carried out, and seeds of G. guangxiense were dealed with 98% H2SO4, different solution concentration of NaOH ,low temperature stratification treatment ,dry storage treatment, different solution concentration treatment of GA3 and 6-BA, so as to discuss the influence of different treatment on seeds germination of G. guangxiense. The results showed that the fresh seeds viability of G. guangxiens was 98% ,however seeds germination of it was just 11.7% when they were cultivated under 5 constant temperature and one alternating temperature, which indicated that there was obvious dormancy phenomenon on fresh seeds of G. guangxiens. Seed coat of G. guangxiens inhibited water absorbtion of seeds, and isolated embryos germination test indicated that in-vitro embryo of seeds havent undergo dormancy,which suggested that seed coats were the main cause that inhibited the seed germination. The tests of breaking seed dormancy showed that dormancy of seeds could not be broken by both low temperature stratification and dry storage method, and the treatment of 98% H2SO4 and different solution concentration of NaOH. Only the treatment of 6-BA with 4 4℃ stratification could heighten the germination rate of G. guangxiens seeds, but the effect of promotion was very poor.
出处
《种子》
CSCD
北大核心
2012年第7期40-43,共4页
Seed
基金
绞股蓝属药用植物种质资源收集保育与DNA条形码研究(编号:桂科攻11107010-3-3)
关键词
广西绞股蓝
种子
休眠
发芽率
Gynostemma guangxiense X. X. Chen et D. H. Qin
seed
dormancy
germination rate
