摘要
目的建立夜明颗粒中肉桂酸、桂皮醛、丹皮酚、23-乙酰泽泻醇B、苍术素醇和苍术素的含量测定方法。方法采用反相高效液相色谱法,色谱柱为Agilent Eclipse XDB-C18(4.6 mm×250 mm,5μm);乙腈-0.3%磷酸梯度洗脱,检测波长240 nm,流速1.0 mL·min-1,柱温30℃。结果肉桂酸、桂皮醛、丹皮酚、23-乙酰泽泻醇B、苍术素醇和苍术素分别在0.309 6～2.322、10.24～76.8、37.36～280.2、0.512 8～3.846、1.612～12.09、10.26～76.95μg.mL-1内与峰面积呈良好的线性关系,其线性回归方程分别为:ρ=0.005 5A+0.008 3(r=0.999 6),ρ=0.025 5A+0.133 6(r=0.999 9),ρ=0.040 8A+0.664 3(r=0.999 7),ρ=0.003 3A+0.017 1(r=0.999 9),ρ=0.016 3A+0.107 5(r=0.999 8),ρ=0.022 9A+0.088 4(r=0.999 9),加样回收率(n=9)分别为100.1%、99.8%、100.7%、99.3%、100.4%和100.5%。结论该方法操作简便、快速,准确度高,重复性好,稳定可靠,可作为夜明颗粒质量控制的方法。
OBJECTIVE To develop a RP-HPLC method for determination of einnamic acid, einnama ldehyde, paeonol, alisol B 23 acetate, atractylodes lancea, and atractylodin in Yeming granules. METHODS The determination was performed on an Eclipse XDB-C18column （4. 6 mm ×250 mm,5 μm） at 30 ℃ with a flow rate of 1.0 mL·min^-1. The mobile phase was 0. 3% phosphate- acetonitrile at gradient elution. The detection wavelength was set at 240 nm. RESULTS The linear regression equations for cinnamic acid, cinnama ldehyde, paeonol, alisolB 23 acetate, atractylodes lancea, and atraetylodin were ρ = 0. 005 5A ＋ 0. 008 3 （ r = 0. 999 6）,ρ=0.0255A＋0.1336 （r=0.9999）,ρ=0.0408A＋0.6643 （r=0.9997）,ρ=0.003 3A＋0. 0171（r=0.9999）,ρ= 0. 016 3A ＋ 0. 107 5 （r =0. 999 8 ） ,ρ=0. 022 9A ＋ 0. 088 4 （r = 0. 999 9 ）, respectively. Good linearity was obtained over the ranges of 0. 309 6 - 2. 322, 10. 24 - 76. 8, 37. 36 - 280. 2, 0. 512 8 - 3. 846, 1. 612 - 12. 09 and 10. 26 - 76. 95 μg· mL^-1, respectively. The recoveries were 100. 1% , 99. 8%, 100. 7% , 99. 3% , 100.4% and 100.5%, respectively. CONCLUSION The method is simple and suitable to control the quality of Yeming granules.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2012年第15期1242-1245,共4页
Chinese Pharmaceutical Journal
基金
吉林省科技厅青年基金项目(20100140)
