摘要
目的:研究6种不同栽培品种南瓜(金钩、甜面、日本、超甜蜜本、蜜本、辽宁新民金钩)的体外抗氧化活性。方法:采用清除二苯代苦味酰基(DPPH)自由基、清除[2,2'-联氨-(3-乙基苯并噻唑啉-6-磺酸)二氨盐](ABTS)自由基及铁离子还原/抗氧化能力(FRAP)测定法,评价南瓜体外抗氧化活性,并与阳性对照二丁基羟基甲苯(BHT)比较。结果与结论:南瓜体外抗氧化活性比较弱。六种样品中,超甜蜜本乙酸乙酯提取部分清除DPPH自由基(抑制率=38.99%)、ABTS自由基(IC50=20.90μg/mL)及还原Fe3+的能力(TEAC=(459.40±10.44)μmol/g)较好,抗氧化活性最强,但仍低于阳性对照BHT(清除率为93.93%,IC50=7.72μg/mL,TEAC=(1581.68±97.41)μmol/g)。
Objective：To research antioxidant activity of 6 varieties of Cucurbita moschata Duch.（Jingou,Tianmian,Riben,Chaotianmiben,Miben and Liaoningxinminjingou）in vitro.Methods：1-diphenyl-2-picrylhydrazyl（DPPH）radical scavenging,[2,2’-azino-bis（3-ethylbenzothiazoline）-6-sulphonic acid]diamonium salt（ABTS）radical scavenging and ferric reducing antioxidant power（FRAP）assay were used to evaluated different varieties of C.moschata with BHT as positive control.Results and Conclusion：Antioxidant activity of C.moschata was not good.The DPPH radical scavenging（I%=38.99%）,ABTS radical scavenging（IC50=20.90μg/mL）,and ferric reducing antioxidant power（TEAC=（459.40± 10.44）μmol/g）of ethyl acetate extract of chaotianmiben C.moschata were higher than others.But it was still inferior to positive control BHT（I%=93.93%,IC50=7.72μg/mL,TEAC=（1581.68±97.41）μmol/g）.
出处
《食品工业科技》
CAS
CSCD
北大核心
2012年第15期90-92,共3页
Science and Technology of Food Industry
基金
河南省科技厅重点攻关项目(112102310310)
河南大学大学生创新性实验计划(11NB021)
