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新城疫病毒活疫苗的制备、活性和临床前安全性试验研究 被引量:1

STUDY OF THE PREPARATION OF NEWCASTLE DISEASE VIRUS VACCINE,ITS ACTIVITY AND TOXICOLOGY EXAMINATION
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摘要 目的 :探讨新城疫病毒活疫苗喷雾吸入剂的简便制备方法及其安全性。方法 :用 SPF种蛋传种、扩增病毒 ,经超速离心等方法提纯后制成疫苗 ;以血凝试验等方法测定其滴度和活性 ;按生物制品的要求进行安全性试验。结果 :本疫苗能保持较高的生物学活性 ,并能达到临床前安全性的内控质量标准要求。结论 :用本法制备供肿瘤临床试验用的 NDV活疫苗是可行的。 Objective:To search for a kind of convenient and in feasible method of preparing Newcastle disease virus (NDV) vaccine Methods:The NDV was vaccinated into allantoic cavity of SPF hen's eggs that have been hatched ten days in 37℃ for virus to replicate and expand in quantity The allantoic liquid containing NDV was collected after vaccination for 72 hours and purified by the ways of superspeed centrifugatization,etc The activity of NDV was detected by the agglutination method by 0 5% cock red blood cells suspension and natural infection of rabbits by spraying the vaccine into nasal cavity mucous membrane The toxicology of NDV was also detected according to standards of biopreparation Results:The NDV vaccine had higher aclirity and could reach the secure requirement of biopreparation quality standards Conclusion:Our method of preparing NDV vaccine was feasible This NDV vaccine could be used for to supplemental therapy of cancer patients by the way of natural infection for effect observation
出处 《广西医科大学学报》 CAS 2000年第1期18-19,共2页 Journal of Guangxi Medical University
基金 广西卫生厅资助课题!(9516)
关键词 新城疫病毒活疫苗 喷雾吸人剂 安全性 肿瘤 NDV live vaccine spray preparation safety
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