摘要
研究尼可地尔、前列地尔及两药物联合应用预处理对心肌缺血再灌注中的影响,探究其作用机制。采用Tunel法检测心肌组织的凋亡情况,免疫组化法检测Bax、Bcl-2和caspase-3的蛋白表达情况。结果显示尼可地尔和前列地尔半剂量联合组细胞凋亡数、Bax和caspase-3的表达量均明显低于其他给药组(P<0.05),Bcl-2的表达量明显高于其他给药组(P<0.05);尼可地尔和前列地尔单给药组细胞凋亡、Bax、caspase-3表达均有降低,Bcl-2表达升高,但效果不及联合用药半剂量组。以此得出结论尼可地尔和前列地尔的联合用药效果较好,可加强对心肌的保护作用。
Investigate the effects of myocardial ischemia and reperfusion when pretreated with nicorandil and alprostadil,and explore the mechanisms. The study tested the apoptosis in tissue with the Tunel method and the expression of Bax,Bcl-2, and caspase-3 protein with immunohistochemistry staining methods. The result showed that the number of apoptosis and the lever of protein expression of Bax, caspase-3 in the group combined nicorandil with alprostadil half dose was lower than others（ P〈0.05）. The group used nicorandil or alprostadil only also liked this, but the degree is lower than the com- bined group. The conclusion is that using nicorandil combined with alprostadil is more effect on protecting the cardiac mus- cle cell.
出处
《医学与哲学（B）》
2012年第7期53-55,共3页
Medicine & Philosophy(B)
关键词
尼可地尔
前列地尔
心肌缺血再灌注
nicorandil, alprostadil, myocardial ischemia reperfusion injury
