摘要
【目的】研究白黄侧耳子实体颜色的遗传规律,为平菇的色泽定向育种提供遗传学依据。【方法】以白黄侧耳Pleurotus cornucopiae白色菌株CCMSSC00358(P-w)和深灰色菌株CCMSSC00406(P-d)为材料,构建自交系2个(P-w×P-w、P-d×P-d),两亲本杂交一代(F1,P-w×P-d),杂交一代中浅灰色和深灰色子实体与二亲本的回交系4个(F1浅×P-w、F1浅×P-d,F1深×P-w、F1深×P-d),共7个家系群体。出菇期观测统计各家系群体不同颜色的个体数。【结果】菌株P-w的自交系出菇29个,子实体全部为白色。菌株P-d的自交系出菇17个,子实体浅灰色5个、深灰色12个,经卡方检验,浅灰色﹕深灰色=1﹕3。杂交一代(F1,P-w×P-d)出菇82个,子实体颜色呈现由浅向深的连续分布,无白色子实体出现。按照深浅分为浅灰色和深灰色两大类,分别为47个和35个,经卡方检验,比例为1﹕1。在回交系中,F1代与白色亲本杂交,经卡方检验,出现深灰色﹕浅灰色﹕白色=1﹕4﹕3的分离;与深灰色亲本杂交,无白色子实体出现,经卡方检验深灰色﹕浅灰色=5﹕3。【结论】白黄侧耳子实体的颜色性状为数量性状,深灰色对白色呈不完全显性,由不同位点上的两对主基因控制。
[Objective] The inheritance analysis of the fruit-body color was carried out in this study to conduct the color breeding of Pleurotus cornucopiae.【Method】White CCMSSC00358（P-w） and dark grey CCMSSC00406（P-d） were used as dikaryon parents.Seven ancestry lines were constructed,respectively,from the two dikaryon parents,two inbred lines P-w×P-w and P-d×P-d,hybrid line P-w×P-d（F1）,two light grey backcross lines F1Light×P-w and F1Light×P-d,two dark grey backcross lines F1Dark×P-w and F1Dark×P-d.The fruit-body color of all the lines was observed and grouped for the statistics of inheritance analysis.【Result】All of the 29 offsprings of inbred line P-w generated white fruit-bodies.Within the 17 offsprings of inbred lines P-d,there were 5 light grey and 12 dark grey offsprings.The segregation of light grey to dark grey was 1：3 checked by Chi-square test.Fruit-body color showed a continuous distribution among 82 offsprings of hybrid generation,and no white fruit-bodies.A total of 47 light grey offsprings and 35 dark grey offsprings were occurred,respectively,and the segregation ratio was 1：1 checked by Chi-square test.The ratio of dark grey,light grey and white was 1：4：3 in offsprings of the backcross（F1×P-w） checked by Chi-square test.The ratio of dark grey to light grey was 5：3 checked by Chi-square test,and no white fruit-body occurred in offsprings of the backcross（F1× P-d）【Conclusion】The color is quantitative trait which may be controlled by two pairs of major genes on different loci,and dark grey is incompletely dominant to white in color for P.cornucopiae.
出处
《中国农业科学》
CAS
CSCD
北大核心
2012年第15期3124-3129,共6页
Scientia Agricultura Sinica
基金
现代农业产业技术体系建设专项资金(CARS-24)
关键词
平菇
品质育种
数量性状
oyster mushroom
quality breeding
quantitative trait
