摘要
目的评价高效液相色谱测定人血清中拉莫三嗪浓度的方法。方法用Hypersil C18色谱柱,柱温为30℃,流动相为1%三(羟甲基)氨基甲烷-乙腈(70∶30),流速为0.8 mL.min-1,测定波长为230 nm。结果拉莫三嗪血清样品线性范围为0.39～50.00 mg.L-1,1.56,6.25,25.00 mg.L-13个浓度质控样品日内、日间精密度(RSD)均<5%,方法学回收率分别为(103.8±3.6)%,(100.0±2.2)%,(99.1±4.2)%。结论本方法回收率高、准确度好、操作简便,适用于临床上对拉莫三嗪血药浓度的常规监测。
Objective To establish a HPLC method of determination of lamotrigine
concentration in serum.Methods Hypersil-C18 column was used,temperature
of colum was 30 ℃,mobile phase was 1% Tris（hydroxymethyl）
aminomethane-acetonitrile（70∶30）,flow rate was 0.8 mL·min-1.The
detecting wavelength was at 230 nm.Results The linear range of
lamotrigine serum samples was 0.39%-50.00 mg·L-1.The intra-day and
inter-day precisions of 3 different concentrations（1.56,6.25,25.00 mg·
L-1） were all less than 5%.The methodology recoveries were（103.8±
3.6）%,（100.0±2.2）%,（99.1±4.2）%,respectively.Conclusion The method
with high recovery,good accuracy,easy operation is suitable for
clinical routine monitoring for serum concentrations of lamotrigine.
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2012年第8期603-605,共3页
The Chinese Journal of Clinical Pharmacology
