摘要
[目的]研究复合酶超声波法提取半边莲生物碱的最佳工艺条件。[方法]以乙醇水溶液为提取剂,采用单因素试验及L16(45)正交试验考察纤维素酶用量、果胶酶用量、酶解温度、pH值和乙醇浓度等5个因素对半边莲中生物碱提取效果的影响。[结果]最佳提取工艺为:纤维素酶用量为24 mg,果胶酶用量为45 mg,酶解时间为30 min,pH值为5.4,乙醇浓度为70%;在此条件下,生物碱的含量为0.860%。[结论]该方法提取率高、稳定性好,可用于半边莲生物碱的提取。
[ Objective ] To study the optimal technical conditions of extracting alkaloids from HERBA LOBEL1AE CHINENSIS with complex enzymes under ultrasonic wave. [ Methods] With ethanol as the solvent, single factor experiment and ortbogonal test method were employed to test the effects of five factors on extraction of alkaloids, including the amount of cellulase, pectinase dosage, extraction time, pH value, and ethanol concentration. [ Results] The optimal extraction procedure was as followed： cellulase 24 mg, pectinase 45 mg, extraction time 30 min, enzymatic pH 5.4, and ethanol concentration 70%. Under these conditions, the alkaloid content is 0. 860%. [ Conclusion] High in ex- traction rate and good in stability, the method favored the determination of alkaloids from HERBA LOBELIAE CHINENSIS.
出处
《安徽农业科学》
CAS
2012年第24期12014-12016,共3页
Journal of Anhui Agricultural Sciences
基金
广西教育厅科研立项项目(编号:201010LX457)
