摘要
以水溶性生物高分子牛血清白蛋白(BSA)为载体,与小分子2,4-二羟基水杨醛缩甘氨酸希夫碱金属配合物(SalGlyM,M=Cu、Zn)结合,制得了水溶性生物高分子金属配合物结合体(SalGlyM@BSA)。采用FT-IR、UV-Vis、CD和SDS-PAGE电泳对其结构进行了表征。采用NBT光化学还原法考察了SalGlyM@BSA的抗O2.-活性,探讨了金属配合物结合体清除O2.-的机理。结果表明氨基酸希夫碱具有一定的抗O2.-活性,结合生物高分子载体后,SalGlyM@BSA具有良好的水溶性和O2.-清除能力。
The water-soluble biopolymer/metal conjugates （ SalGlyM@ BSA） were prepared by the small molecule metal complexes, 4-hydroxy-salieylaldehyde Glyeine Schiff-base metal complexes （ SalGlyM, M = Cu Zn, conjugating with water-soluble biopolymer, bovine serum albumin（BSA）. The structure of SalGlyM@ BSA were characterized by FT-IR spectra, UV-Vis spectra, circular dichroism（CD） and SDS-PAGE electrophoresis. The superexide anion radical （ O2.-） scavenging activity of SalGlyM, BSA and SalGlyM@ BSA complexes were investigated by NBT photochemical reduction and the effect mechanism of scavenging O2.- was also explored. The results showed that Glycine Schiff-base complexes had a certain antioxidant activity. After combining SalGlyM into BSA,the biopolymer/metal conjugates （SalGlyM@ BSA）showed water solubility and excellent O2.- scavenging activity.
出处
《化学研究与应用》
CAS
CSCD
北大核心
2012年第8期1217-1220,共4页
Chemical Research and Application
基金
国家自然科学基金(20964002)
甘肃省高校基本科研业务费专项资金项目(2010-176)
