摘要
目的监测多药耐药菌(MDROs)耐药性,为临床合理用药、有效控制MDROs感染提供依据。方法用法国生物梅里埃公司生产的细菌自动鉴定仪分离鉴定菌株,药敏监测采用珠海迪尔生物工程有限公司生产的DL系产品进行最低抑菌浓度的检测。结果两年中临床科室病原学送检率由2009年的45.39%升至2010年的61.87%(P<0.05),MDROs在病原菌中的构成比由26.83%降至23.56%(P<0.05);检出的746株MDROs,其中革兰阳性菌占49.87%,革兰阴性菌占50.13%;大部分耐>7种常用抗菌药物;常见革兰阳性菌为肺炎链球菌、溶血性葡萄球菌及表皮葡萄球菌,对阿莫西林/克拉维酸、头孢唑林、磺胺甲噁唑/甲氧苄啶等抗菌药物耐药率均>80.00%;主要革兰阴性菌为产ESBLs的大肠埃希菌、肺炎克雷伯菌,多药耐药铜绿假单胞菌,对哌拉西林、头孢噻肟等抗菌药物的耐药率均>50.00%。结论 MDROs对大部分常用抗菌药物耐药,应重视抗菌药物的合理应用,减缓细菌耐药性的增长。
OBJECTIVE To investigate the antimicrobial resistance of multidrug-resistant organism （MDROs） so as to provide bases for the rational use of antimicrobial agents and effective control of MDROs infections. METHODS The strains were isolated and identified by Automatic Identification System Bacteria of Merieux, and the drug susceptibility testing was performed with DL of Deere Biological Engineering Co. , Ltd. ZhuHai. RESULTS The clinical submission rate of bacteriology rose from 45.39 % in 2009 to 61.87 % in 2010 （P〈0.05）, the proportion of MDROs in the pathogens decreased from 26.83% to 23.56% （P〈0.05） ; of 746 strains of MDROs isolated, gram-positive bacteria accounted for 49.87%, gram-negative bacteria accounted for 50. 13% most of the strains were resistant to more than 7 antibiotics; Streptococcus pneumoniae, S. hemolytic and S. epidermidis were most common gram-positive bacteria, the resistance rates to amoxicillin/clavulanic acid, cefazolin, cotrimoxazole were higher than 80. 0%; ESBLs-producing Escherichia coli and Klebsiella pneumonia, multidrug-resistant Pseudo- monas aeruginosa were most common gram-negative bacteria, the resistance rates to piperacillin and cefotaxime were higher than 50.0 %. CONCLUSION MDROs are resistant to the most of common antibiotics, we must attach importance to the rational use of antimicrobial agents so as to restrain the increase of the bacterial resistance.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2012年第16期3636-3638,共3页
Chinese Journal of Nosocomiology
