摘要
目的:对桃金娘茎枝中的没食子酸进行提取分离鉴定并测定含量。方法:采用80%乙醇对桃金娘茎枝进行提取,利用柱层析和重结晶对提取液进行分离,通过理化性质鉴别和波谱技术鉴定其结构;采用高效液相色谱法测定桃金娘茎枝中没食子酸含量,色谱柱:LichrospherC18,流动相:甲醇-0.2%磷酸水溶液(1.5∶98.5),流速1.0mL/min,检测波长为270nm,柱温为室温,进样量为10μL。结果:没食子酸在0.1~0.6μg范围内呈良好线性关系(r=0.9992),平均回收率为100.6%,RSD为1.32%(n=6)。结论:该方法简便可行,重现性好,可用于桃金娘茎枝中没食子酸含量测定。
Objective： To isolate and determine gallic acid in stem and branch of Rhodomyrtus tornentosa（Ait.）Has- sk.. Methods： Gallic acid was extracted by 80% ethanol, isolated and purified by column chromatography, and recrys- tallized from the stem and branch of Rhodomyrtus tornentosa（Ait. ） Hassk.. A HPLC method was set up to determine the content of gallic acid in Rhodornyrtus tornentosa （Ait.）Hassk., using Lichrospher C18 （4.6 mm× 250 mm, 5 μm） column, and methanol-0.2%-phosphoric acid water solution （1.5：98.5） as the mobile phase, the UV detection wavelength was at 270 nm, with the flow rate of 1.0 mL/min at room temperature. Results： A good linear was obtained in the range of 0.1 - 0.6 I^g and the average recovery was 100.6% （RSD =1.32%, n = 6）. Conclusion： The method is simple with a good reproducibility for the determination of gallic acid in Rhodomyrtus tornentosa（Ait. ）Hassk.
出处
《中国执业药师》
CAS
2012年第8期14-16,17,共4页
China Licensed Pharmacist
基金
广西自然科学基金项目(桂科自0640077)
关键词
桃金娘
没食子酸
分离
鉴定
含量测定
Rhodornyrtus tomentosa （Ait.） Hassk.
Gallic Acid
Isolation
Identification
Content Determination
