摘要
目的:优选松花粉中总酚的超声提取工艺。方法:以总酚得率为指标,采用单因素试验考察乙醇体积分数、超声时间、超声温度、料液比等因素对总酚得率的影响,在此基础上,运用响应面法优选总酚超声提取工艺。结果:松花粉总酚的最佳超声提取工艺条件为30倍量39%乙醇于51℃下超声提取24 min,在此条件下,总酚得率3.85 mg.g-1,实测值与模型预测值相符。结论:响应面分析法可较好地用于优化松花粉总酚超声提取工艺,且该优选工艺方便、快捷、高效。
Objective： To optimize ultrasonic-assisted extraction technology of total phenols from Pinus massoniana by response surface methodology. Method： With yield of total phenols as index, the concentration of ethanol, ultrasonic time, ultrasonic temperature and solid-liquid ratio were investigated by single factor test, based on this, ultrasonic extraction technology of total phenols was optimized by response surface methodology. Result： Optimal extraction conditions were： ultrasonic extracted 24 min with 30 times the amount of 39% ethanol at 51 ℃, under these conditions, yield of total phenols reached 3.85 mg·g^-1, which was agreed with model predictions. Conclusion： Ultrasonic-assisted extraction technology was simple, rapid, and efficient, response surface method could used well to optimize ultrasonic-assisted extraction of total phenols from P. massoniana.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第16期53-56,共4页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
松花粉
总酚
超声提取
响应面设计
Pinus massoniana
total phenols
uhrasonic-assisted extraction
response surfacemethodology
