摘要
目的:建立柱前衍生反相高效液相色谱法同时测定驴胶补血颗粒中6种水解氨基酸含量的方法。方法:采用Hypersil BDS C18色谱柱(4.6 mm×200 mm,5μm),异硫氰酸苯酯为柱前衍生剂,梯度洗脱,流速1.0 mL.min-1,检测波长254nm,柱温30℃。结果:L-谷氨酸的线性范围0.169～1.014μg(r=0.999 5),平均回收率101.43%,RSD 3.20%;L-羟脯氨酸的线性范围0.178～1.068μg(r=0.999 9),平均回收率101.23%,RSD 3.25%;甘氨酸的线性范围0.381～2.286μg(r=0.999 5),平均回收率102.87%,RSD 2.32%;L-丙氨酸的线性范围为0.162～0.972μg(r=0.999 5),平均回收率102.37%,RSD 3.36%;L-精氨酸的线性范围0.118～0.708μg(r=0.999 6),平均回收率102.73%,RSD 2.41%;L-脯氨酸的线性范围0.253～1.518μg(r=0.999 9),平均回收率98.69%,RSD 2.34%。结论:建立的方法可靠,可用于驴胶补血颗粒中氨基酸的含量检测。
Objective： To develop a HPLC method for determining 6 amino acids in Lujiao Buxue keli. Method： The sample was derived with phenyl isothiocyanate （PITC）. Amino acids were separated on Hypersil BDS Cls （4.6 mm×200 mm, 5 μm） column at the flow rate of 1.0 mL·min^-1 , detected at 254 nm. Result： L- Glu had good linearity in the range of 0. 169-1. 014 μg （r =0. 999 5）. The average recovery was 101.43% , RSD was 3.20% ; L-Hyp had good linearity in the range of 0. 178-1. 068μg （r = 0. 999 9）. The average recovery was 101.23%, RSD was 3.25%; Gly had good linearity in the range of 0.381-2.286 μg （r =0.999 5）. The average recovery was 102.87% , RSD was 2.32% ; L-Ala had good linearity in the range of 0. 162-0. 972μg （r = O. 999 5）. The average recovery was 102.37%, RSD was 3.36%; L-Arg had good linearity in the ranges of 0.118-0.708μg （r =0.999 6）. The average recovery was 102.73%, RSD was 2.41%; L-Pro had goodlinearity in the range of 0. 253-1. 518μg （r =0. 999 9）. The average recovery was 98.69% , RSD was 2.34%. Conclusion： The method is reliable, which is helpful for the determination of amino acids in Lujiao Buxue keli.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第16期93-96,共4页
Chinese Journal of Experimental Traditional Medical Formulae
