摘要
用聚丙烯酰胺垂直板凝胶电泳技术对北马兜铃营养生长期根、幼茎、老茎、叶柄、未展开幼叶、展开幼叶和成熟叶的过氧化物酶(POD)同工酶进行了分析。结果表明:北马兜铃营养生长期7个组织器官共有10条POD酶带,其POD同工酶具有组织特异性,其中未展开幼叶的POD同工酶酶带丰富、活性较强、含量较高,是POD同工酶分析最好的材料,而成熟叶片由于POD酶种类太少,最不适合作POD同工酶分析。
The POD enzymes from root, tender stem, old stem, petiole, unexpanded tender leaf, expanded tender leaf and mature leaf of Aristolochia contorta Bunge at vegetative growth phase were analyzed by vertical polyacrylamide gel electrophoresis （PAGE）. The results showed that 10 bands of POD isoenzymes from 7 different tissues and organs ofAristolochia contorta Bunge at vegetative growth phase were expressed and POD isoenzymes had a tissue specificity. Unexpanded tender leaf which had abundant enzyme bands, stronger activity and higher content were the good materials for analysis on POD isoenzyme. There were the fewest enzyme bands in mature leaf, so it was not fit for analysis on POD isoenzyme.
出处
《北方园艺》
CAS
北大核心
2012年第16期88-89,共2页
Northern Horticulture
基金
佳木斯大学大学生科技创新资助项目(Dz2011-002)
关键词
北马兜铃
营养生长期
过氧化物酶
同工酶
Aristolochia contorta Bunge
vegetative growth phase
POD
isoenzyme
