摘要
在50 L的生物反应器中,通过控制溶解氧水平为5%、10%、20%、30%四个水平考察枯草芽孢杆菌发酵生产腺苷的影响,发现该菌株生长的溶解氧浓度在10%～20%。并通过发酵过程中菌株的生长情况、菌体摄氧率和发酵产苷进行相关分析。结果表明,在发酵过程中DO水平控制在10%～20%时腺苷积累量高,发酵液中DO水平为5%和30%均不利于发酵液中的腺苷积累。通过对发酵终点丙酮酸的检测,发现枯草芽孢杆菌在低溶氧状态下比高溶氧状态下积累更多的丙酮酸。在此基础上,提出两阶段DO控制策略,最终腺苷积累量达到20.1 g/L。
The effect of oxygen supply on adenosine production of Bacillus subtilis was studied using 50L fermen- tor with the air saturation at 5% , 10% , 20% and 30%. We found that the concentration of the critical dissolved oxy- gen in the industrial strains was 10% - 20%. The relationship among strain growths, oxygen uptake rate in the process and dissolved oxygen （DO） tension on adenosine accumulation was analyzed. The results showed that during the adenosine fermentation of Bacillus subtilis, it was better to control DO at 10% -20% than at 5% and 30%. We found that the concentration of pyruvate at the final time of fermentation was higher under low oxygen condition than that under high oxygen condition. Based on the results, a strategy of two stages for DO manipulation was proposed. With this strategy, the adenosine accumulated in 50L fermentor ultimately reached 20. l g/L.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2012年第7期86-89,共4页
Food and Fermentation Industries
关键词
枯草芽孢杆菌
发酵
腺苷
溶解氧
Bacillus subtilis, fermentation, adenosine, dissolved oxygen
