摘要
目的比较阿托品与盐酸戊乙奎醚对小鼠胃肠激素的影响。方法健康昆明小鼠30只,体重18～24g,雌雄不拘,随机均分为三组后分别腹腔注射等容量等渗盐水(对照组,Ⅰ组)、阿托品0.3mg/kg组(Ⅱ组)、盐酸戊乙奎醚0.3mg/kg组(Ⅲ组)。三组给药30min后摘取眼球取血,处死小鼠,采用放免法测定血浆和胃肠组织中的胃动素和血管活性肠肽水平。结果Ⅱ组中小鼠血浆与组织中的血管活性肠肽水平明显高于Ⅰ、Ⅲ组(P<0.05),而胃动素水平则明显低于Ⅰ、Ⅲ组(P<0.05);Ⅰ、Ⅲ组差异无统计学意义。结论阿托品影响血管活性肠肽及胃动素的分泌,而盐酸戊乙奎醚则无影响。
Objective To compare the effect of penehyelidine hydrochloride and atropine on gastrointestinal hormone in mice. Methods Thirty healthy Kunming mice of both sexes weighing 18- 24 g were randomly divided into three groups（n= 10）： group I received equi-volume normal saline, group Ⅱ received 0. 3 mg/kg of atropine intraperitoneally and group Ⅲ received 0. 3 mg/kg of penehyclidine hydrochloride intraperitoneally. The blood was collected at 30 min after the injection, and then sacrificed the animal for tissue collection. The contents of motilin and vasoactive intestinal peptide in plasma and gastrointestinal tissue were detected with： radioimmunoassay. Results Compared with group Ⅲ, the level of vasoactive intestinal peptide in both plasma and gastrointestinal tissue was significantly decreased in groups Ⅰ and Ⅲ, but the level of motilin was markedly increased （P 〈 0.05）. No significant difference was observed in groups Ⅰ and m. Conclusion Atropine influences the excretion of vasoactive intestinal peptide and motilin, but penehyclidine hydrochloride not.
出处
《临床麻醉学杂志》
CAS
CSCD
北大核心
2012年第7期703-704,共2页
Journal of Clinical Anesthesiology
关键词
盐酸戊乙奎醚
血管活性肠肽
胃动素
阿托品
Penehyclidine hydroehloride
Vasoactive intestinal peptide
Motilin
Atropine
