摘要
目的探讨应用蛋白质芯片技术诊断伤寒、副伤寒的可行性。方法利用蛋白质芯片方法,将伤寒抗原(To、Th)和副伤寒抗原(Ta、Tb、Tc)及伤寒Vi荚膜多糖抗原集成到已经活化处理的玻璃芯片上,使其保持蛋白质活性和立体结构不变。对86例经血培养确认的已知标本和100例健康体检标本进行检测,并与肥达氏反应作比较。结果蛋白质芯片法检测结果与血培养结果符合率高,灵敏度为97.6%,特异度为98.0%,而肥达氏反应灵敏度为60.4%,特异度98.0%,两方法之间差异有统计学意义(P<0.01)。结论蛋白质芯片法具有高通量、可并行检测伤寒、副伤寒多种抗体的优势,为伤寒、副伤寒的诊断提供了有效手段。
Objective To evaluate a new protein microarray method for the diagnosis of typhoid and paratyphoid fever.Methods Salmonella typhi antigens To and Th,Salmonella paratyphi antigens Ta,Tb and Tc,and Vi capsular polysaccharide of Salmonella typhi were spotted on glass slides,which were modified with specific active groups.A total of 86 cases of known samples,confirmed by blood culture,and 100 cases of samples from healthy subjects were detected by constructed method,and the detection results were compared with those of Widal test.Results There was a high consistence between protein microarray and blood culture.The sensitivity and specificity of protein microarray were respectively 97.6% and 98.0%,higher than 60.4% and 98.0% of Widal test（P〈0.01）.Conclusion The protein microarray technology might with more advantages than Widal test and blood culture,and it be with much potentials to apply in clinical diagnosis.
出处
《国际检验医学杂志》
CAS
2012年第12期1438-1439,1441,共3页
International Journal of Laboratory Medicine
