摘要
目的建立简单有效的高原实地多脏器功能障碍综合征（MODS）绵羊模型。方法绵羊随机分为对照组、实验I组和实验II组，全麻后，3mg／kg和6mg／kg内毒素（LPS）分别于30min和60min内静脉泵入实验I组和实验II组绵羊，对照组以相同方法泵入生理盐水。以不同时点测定血气、白细胞数目和血清生化的变化。结果实验I组和II组绵羊的死亡率分别为50％和70％；3h内氧合指数明显降低（P〈O．05或P〈0．01）；不同时点肌酸激酶（CK）、肌酸激酶同工酶（CK—MB）、天门冬氨酸转氨酶（AST）、丙氨酸氨基转移酶（ALT）、总胆红素（TBIL）和肌酐（CRE）明显升高（P〈0．05或P〈0．01），而CK和CK．MB在48h～72h时则明显低于对照组（P〈O．05或P〈0．01）：白细胞（WBC）数目在3h明显降低，之后又明显升高（P〈O．05或P〈0．01）。结论两种剂量的LPS均能成功地建立高原实地绵羊MODS模型。
Objective To establish ansimple and effective animal model of multiple organ dysfunction syndrome （MODS） induced by lipopolysaccharide （LPS） on highland scene in sheep. Methods Sheep was divided into the control, Group I and Group. After anaesthetizing, 3 mg/kg and 6 mg/kg LPS were intravenously infused into Group I and Group II sheep in 30 minutes and 60 minutes respectively. Sheep in the control group was treated by isotonic Na chloride in the same method. Then the indexes were determined in blood gas, the number of white blood cell （blood routine WBC） and blood biochem- istry at the different time point. Results After intravenous injecting LPS, the death rate was 50% and 70% in Group I and Group II respectively. The oxygenation index was evidently descendent （P〈0.05 or P〈0.01）, creatine kinase （CK）, creatine kinase isozyme （CK-MB）, aspartic transaminase （AST）, alanine aminotransferase （ALT）, total bilirubin （TBIL） and creatinine （CRE） were conspicuously ascendant （P〈0.05 or P〈0.01） at the different time point. But CK and CK-MB were obviously descendent （P〈0.05 or P〈0.01） from 48 h to 72 h. The number of white blood cell （WBC） was obviously decreased in 3 hours after intravenous injecting LPS, then it markedly increaed （P〈0.05 or P〈0.01）. Conclusion The animal model of MODS was established by 3 mg/kg and 6 mg/kg of LPS successfully on highland scene.
出处
《实验动物与比较医学》
CAS
2012年第4期285-289,共5页
Laboratory Animal and Comparative Medicine
基金
甘肃省自然科学基金（No.1010RJZA052）
关键词
高原
多脏器功能障碍综合征
内毒素
动物模型
Highland scene
Multiple organ dysfunction syndrome
Lipopolysaccharide
Animal model
