摘要
以石蒜鳞茎为材料,建立了石蒜生物碱的反相高效液相色谱(HPLC)分析方法。探讨了流动相中乙腈含量、三乙胺含量、pH值、流速等对生物碱分离的影响,结果表明在ZORBAX ODS-C18(150 mm×4.6mm,5μm)反相色谱柱上,以0.9%三乙胺水溶液(pH 8.0)-乙腈-甲醇为流动相,梯度洗脱,流速为1mL/min,检测波长为234 nm时,石蒜样品可有效分离出14种石蒜生物碱,分离度良好。加兰他敏、力可拉敏和石蒜碱的检出限分别为3.375、0.475、0.495 mg/L,平均加标回收率均为98%。运用此法测定了石蒜不同部位中3种生物碱的含量,结果表明石蒜不同部位的3种生物碱含量差异较大,叶子中的含量最高;同一部位中,石蒜碱的含量最高,力可拉敏次之,加兰他敏最低。
A reversed-phase high performance liquid chromatographic method was developed for the determination of three alkaloids of Lycoris radiate.Chromatographic separation was performed on a ZORBAX ODS-C18 reversed phase column（150 mm × 4.6 mm,5 μm）.Effects of mobile phase,pH and flow rate on the analysis of alkaloids of Lycoris radiate were investigated.The optimized chromatographic conditions for the alkaloids of Lycoris radiate were as follows：mobile phase：0.9% triethylamine aqueous solution（pH 8.0 adjusted with acetic acid）（A）-acetonitrile（B）-methanol（C）,flow rate：1.0 mL /min,detection wavelength：234 nm.Under the optimal conditions,14 alkaloids were successfully separated from Lycoris radiate sample.The detection limits were 3.375 mg/L for galanthamine,0.475 mg/L for lycoramine and 0.495 mg/L for lycorine,respectively.The average recoveries were all 98%.The method was applied in the detection of three alkaloids contents in different parts of Lycoris radiate.The result indicated that the contents were significantly different in different parts.The highest content part of alkaloids was the leaves of Lycoris radiate.In the same part,the content of lycorine was the highest among them three,and that of galanthamine was the lowest.
出处
《分析测试学报》
CAS
CSCD
北大核心
2012年第8期957-961,共5页
Journal of Instrumental Analysis
基金
安徽省自然科学基金(1208085mc48)
国家科技支撑计划课题资助(2009BADA6B06
2008BAD96B12)
关键词
石蒜
高效液相色谱
加兰他敏
力可拉敏
石蒜碱
Lycoris radiate
high performance liquid chromatography
galanthamine
lycoramine
lycorine
