摘要
目的:探讨周围组织和骨水泥表面粗糙度两因素对诱导膜组织学特性的影响。方法:将表面光滑和粗糙的两种骨水泥分别植入兔子桡骨缺损、肌肉内和皮下,在体内形成诱导膜。6周后取诱导膜组织行HE染色,光镜下获取诱导膜组织学图片,并在图片自动分析软件中对诱导膜的厚度、面积、细胞密度、细胞外基质密度、微血管密度进行定量评估。采用两因素方差分析评价周围组织和骨水泥表面粗糙度2个因素对诱导膜各个参数的影响。结果:组织学观察两种骨水泥在3个部位均能形成诱导膜。诱导膜组织学特性在桡骨缺损、肌肉内和皮下3个部位有所差异:差异表现在膜厚度、面积及微血管密度3个参数的不同(P=0.000,P=0.000,P=0.000);而细胞密度和细胞外基质密度2个参数无明显差异(P=0.734,P=0.638)。表面粗糙度不同的骨水泥在相同组织内诱导形成的膜具有相似的组织学特性,其5个组织学参数均无明显差异(P=0.506,P=0.185,P=0.883,P=0.093,P=0.918)。结论:周围组织对形成的诱导膜组织学特性有明显的影响,而骨水泥表面粗糙度对诱导膜组织学特性的影响并不明显。组织中的纤维细胞数量、血供、机械张力及微动环境可能是影响诱导膜组织学特性的因素。
Objective ：To explore surface roughness of bone cement and surround tissue on histological characteristic of induced membranes. Methods：Bone cements with smooth and rough surface were implanted in radius bone defect, intramus- cular and subcutaneous sites of rabbits, and formed induced membranes. Membranes were obtained and stained （HE） 6 weeks later. Images of membrane tissue were obtained and analyzed with an automated image analysis system. Five histological pa- rameters of membranes were measured with thickness, area, cell density,ECM density and microvessel density. Double factor variance analysis was used to evaluate the effect of the two factors on histological characteristics of induced membranes. Re- suits： Membranes can be induced by each kind of bone cement and at all the three tissue sites. In histological parameters of thickness, area and micro vessel,there were significant differences among the membranes induced at different tissue sites （P=- 0.000,P=0.000,P=0.000） ;whereas,there were no significant differences in histological parameters of cell density and ECM density （P=0.734, P=0.638 ）. In all five histological parameters of membranes, there were no significant differences between the membranes induced by bone cements with different surface roughness （P=0.506, P=0.185, P=0.883, P=0.093, P=0.918 ）.Con- clusion： Surround tissue rather than surface roughness of bone cements can affect the histological characteristics of induced membranes. The fibrocystic number,vascularity, mechanical tension and micro motion of the surround tissue may be closely correlated with the histological characteristics of induced membranes.
出处
《中国骨伤》
CAS
2012年第8期662-666,共5页
China Journal of Orthopaedics and Traumatology
基金
国家自然科学基金项目(面上项目)(编号:31060135/C100302)
浙江省医药卫生科学研究基金(编号:2007B147)~~
关键词
皮下组织
肌肉
骨缺损
骨水泥
组织学
Subcutaneous tissue
Muscles
Bone defect
Bone cement
Histology
