摘要
以脱脂鮟鱇鱼肝为原料,以水解度及对羟自由基的清除活性为考察指标,用木瓜蛋白酶、碱性蛋白酶、中性蛋白酶、胃蛋白酶及胰蛋白酶进行单酶解筛选试验。以水解度及对羟自由基的清除作用为指标,应用二次正交旋转组合设计试验研究加酶量、酶解时间、pH值及温度对制备鮟鱇鱼肝抗氧化肽工艺的影响。综合考虑水解度和对羟自由基的清除活性因素,最终确定碱性蛋白酶酶解鮟鱇鱼肝制备抗氧化肽的最佳工艺条件是:加酶量3000U/g,酶解时间6h,pH8.5,酶解温度55℃。该条件下制备的鮟鱇鱼肝抗氧化肽产物水解度和对羟自由基的清除分别为69.52%、76.74%。
Preparation of Lophius litulon liver active peptide by five proteases and its hydroxyl radical scavenging activities were studied. The results show that Alcalase is a perfect protease to prepare Lophius litulon liver active peptide. Effect of enzyme dosage, hydrolysis time, hydrolysis temperature, pH value on preparation of Lophius litulon liver active peptide with Alcalase were studied by single factor analysis. The regression models were established with factors and the indexes, including of degree of hydrolysis and hydroxyl radical scavenging activities. The results indicate that optimal hydrolytic conditions for preparation of Lophius litulon liver active peptide by Alcalase are as follows： enzyme dosage 3 000 U/g, hydrolysis time 6 h, hydrolysis temper ture 55 ℃, pH value 8.5. Under this condition, the DH is 69.52% and hydroxyl radical scavenging activities is 76.74%.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2012年第7期9-16,共8页
Journal of Chinese Institute Of Food Science and Technology
基金
国家自然科学基金资助项目(31071628)
国家国际科技合作项目(2012DFA30600)
国家科技支撑计划项目(2012BAD29B06)
舟山市海洋类科技计划项目(092018)
关键词
鮟鱇鱼肝
水解
抗氧化肽
羟自由基
二次回归正交旋转组合设计
Lophius litulon liver
hydrolysis
antioxidant peptide
hydroxyl radicals
quadratic regression orthogo-nal ratating combination design
