摘要
目的 :探讨子宫内膜异位症白细胞介素 8(IL 8)、白细胞介素 10 (IL 10 )水平及中药内异方对其调节作用。方法 :根据CummingsAM方法建立子宫内膜异位症动物模型 ,随机分成非治疗组、内异方组、三苯氧胺组 ,另设正常对照组。采用ELISA法检测IL 8、IL 10含量。结果 :非治疗组外周血及腹腔液IL 8水平、腹腔巨噬细胞数量显著高于正常对照组 ;内异方组异位种植内膜组织重量、腹腔巨噬细胞数量均显著低于非治疗组 ;非治疗组腹腔巨噬细胞在脂多糖刺激下产生的IL 10含量显著低于正常对照组和内异方组。结论 :子宫内膜异位症细胞因子IL 8、IL 10等的异常与疾病的发生发展有关。内异方通过促进IL 10的分泌 ,从而抑制一系列炎症介质及细胞因子的合成 ,阻止了异位内膜组织的生长。
To investigate the IL 8 and IL 10 levels in endometriosis and the regulated role of Neiyifang (NYF) on them. Methods: Animal models with endometriosis after Cummings method was established. IL 8 and IL 10 levels were determined in using double antibody sandwich ELISA. Results: The IL 8 levels of serum and peritoneal fluid, and peritoneal macrophage amounts were markedly higher in the untreated group than those in the control group. The weights of endometriotic tissue and peritoneal macrophage amounts were markedly lower in the treated group of NYF, compared with the untreated group. The lipopolysaccharide stimulated IL 10 secretion in peritoneal macrophages from the untreated group were significantly lower compared with the control group and the treated group of NYF. Conclusion: The abnormality of IL 8 and IL 10 levels in endometriosis may be related to the pathogenesis of the disease. The therapeutical mechanism of NYF was mediated by promotion of IL 10 secretion, and further restrained the composition of inflammatory media and the growth of endometriotic tissue.
出处
《中国中西医结合杂志》
CSCD
北大核心
2000年第8期603-605,共3页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家自然科学基金!资助项目 (No .3960 0 1 95)
国家中医药管理局基金!资助项目 (No.95Y2 31 3)
