摘要
目的:探求一种合理有效的研究中药作用于中枢神经系统药效的方法。方法:利用体外细胞培养的方法,以三种不同浓度观察血清、中药血清、脑脊液、中药脑脊液对于星形胶质细胞和神经元的影响。结果:台盼兰染色和LDH检测证明正常血清和中药血清在三种浓度均出现对细胞的毒害作用(P<0.05),尤以100μl、200μl为重,且在同一浓度时,两组之间没有明显的差异(P>0.05)。而正常脑脊液组和中药脑脊液组与空白对照组相比,未见明显差异(P>0.05);MTT检测证明中药脑脊液组OD值明显高于其余各组(P<0.05)。结论:利用脑脊液进行中药对中枢神经系统的药效观察明显优于血清,提出“脑脊液药理学”的概念;中药可诱导星形胶质细胞产生神经营养作用。
Objective: To find a effective method by which the potency of Chinese materia medica (CMM) on the central nervous system (CNS) could be correctly investigated. Method: The cerebrospinal fluid(CSF) and serum after oral CMM 1 to 1.5 hours was gained and then added into the astrocyte cell culture medium. After 48 h, the above medium was in proportion transferred to the neuron culture medium. Typan blue stainning, LDH and MTT test were utilized to observe the drug effect on the neuron survival. Results: The serum, but not the CSF, showed the toxicity on the astrocyte. MTT test on the neuron survival showed that the OD492nm value in the CMM group was higher than the other groups (P < 0.05). The astrocyte medium stimulated by the CSF including CMM could promote the neuron axon growth. Conclusion: If observing the CMM potency on CNS in vitro, the CSF was better than the serum, we called it'Cerebrospinal Fluid Pharmacology in Chinese Materia Medica'. Furthermore, the CMM could induce the neurotrophy of astrocyte.
出处
《中药材》
CAS
CSCD
2000年第8期467-470,共4页
Journal of Chinese Medicinal Materials
基金
国家九五科技攻关项目(96-906-09-03)
